Plant Pathology Department, University of Florida, Gainesville, FL 32611, U.S.A.
North Florida Research and Education Center, University of Florida, Quincy, FL 32351, U.S.A.
Phytopathology. 2024 Sep;114(9):2033-2044. doi: 10.1094/PHYTO-11-23-0449-R. Epub 2024 Sep 16.
Bacterial spot caused by spp. is an economically important disease of pepper causing significant yield losses in Taiwan. Monitoring the pathogen population on a continuous basis is necessary for developing disease management strategies. We analyzed a collection of xanthomonad strains isolated from pepper in Taiwan between 1989 and 2019. Among the sequenced genomes, 65 were identified as , and 10 were . Thirty-five and 10 strains were copper tolerant, whereas only four and none of the strains were tolerant to streptomycin. Nine strains were amylolytic, which is considered an unusual characteristic for . Bayesian analysis of the population structure based on core gene single-nucleotide polymorphisms clustered the strains into five clusters for and three clusters for . One cluster, designated as TP-2019, appears to be a novel genetic cluster based on core genes, accessory gene content, and effector profile. This knowledge of pathogen diversity with whole genomic information will be useful in future comparative studies and in improving breeding programs to develop disease-resistant cultivars and other disease management options.
由 spp.引起的细菌性斑点病是一种对辣椒具有重要经济意义的疾病，在台湾造成了显著的产量损失。为了制定病害管理策略，有必要对病原体种群进行持续监测。我们分析了 1989 年至 2019 年间从台湾辣椒中分离出来的一组黄单胞菌菌株。在测序的基因组中，有 65 个被鉴定为 ，10 个为 。35 个 和 10 个菌株对铜具有耐受性，而只有 4 个 菌株和 个 菌株对链霉素具有耐受性。9 个 菌株具有淀粉水解能力，这被认为是 的一个不寻常特征。基于核心基因单核苷酸多态性的种群结构贝叶斯分析将菌株聚类为 5 个簇和 3 个簇。一个 菌株簇，命名为 TP-2019，根据核心基因、辅助基因含量和效应子图谱，似乎是一个新的遗传簇。这种基于全基因组信息的病原体多样性知识将有助于未来的比较研究，并有助于改进育种计划，以开发抗病品种和其他病害管理方案。
