ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India; College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan, India.
College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan, India.
Cryo Letters. 2024 Jul-Aug;45(4):257-268.
Little is known about the effects of different seasons on the cryopreservation success of buffalo sperm in terms of kinematics and sperm functional parameters.
To study the effect of three seasons (winter, comfort and summer) and cryopreservation on sperm kinematics and functional properties in buffalo bulls.
Semen ejaculates (n = 90) collected during three seasons i.e. winter (n = 30), comfort (n = 30), summer (n = 30) were evaluated for sperm kinematics and functional properties.
Sperm kinematics with respect to total (TM), progressive (PM) and rapid motility (RM) was higher (P < 0.05) in fresh sperm compared to sperm that had been frozen-thawed. Similarly, all kinematic parameters [viz. average path velocity (VAP), straight linear velocity (VSL), curvilinear velocity (VCL), beats cross frequency (BCF), lateral head displacement (ALH), linearity (LIN) and straightness (STR)] were higher (P < 0.01) at the fresh stage. With respect to season, frozen-thawed semen TM (57.67 ± 115 %), PM (50.2 ± 1.15 %) and RM (51.6 ± 1.19 %) were higher (P < 0.01) when using sperm collected during winter. The stage of cryopreservation (i.e., equilibration and freeze-thawing) also showed significant effects (P < 0.01) on mitochondrial superoxide positive status (MSPS), mitochondrial membrane potential (MMP), acrosome status and intra-cellular calcium status.
The season of sperm collection and cryopreservation have significant effects on buffalo bull sperm kinematics and functional properties. Doi.org/10.54680/fr24410110612.
关于不同季节对水牛精子冷冻保存成功率的影响，鲜为人知，包括运动学和精子功能参数方面。
研究三个季节（冬季、舒适季和夏季）和冷冻保存对水牛公牛精子运动学和功能特性的影响。
在三个季节（冬季、舒适季和夏季）采集精液样本（n=90），评估精子运动学和功能特性。
与新鲜精子相比，冷冻-解冻后的精子总活力（TM）、前向运动活力（PM）和快速直线运动活力（RM）更高（P<0.05）。同样，所有运动学参数[即平均路径速度（VAP）、直线速度（VSL）、曲线速度（VCL）、鞭打频率（BCF）、侧向位移（ALH）、直线性（LIN）和直线性（STR）]在新鲜阶段更高（P<0.01）。就季节而言，冬季采集的冷冻-解冻精子的 TM（57.67±115%）、PM（50.2±1.15%）和 RM（51.6±1.19%）更高（P<0.01）。冷冻保存阶段（即平衡和冷冻-解冻）也对线粒体超氧化物阳性状态（MSPS）、线粒体膜电位（MMP）、顶体状态和细胞内钙状态有显著影响（P<0.01）。
精子采集和冷冻保存的季节对水牛公牛精子运动学和功能特性有显著影响。
