Han Chuang, Cheng Qin, Du Xiaoping, Liang Lianming, Fan Guocheng, Xie Jiatao, Wang Xu, Tang Yanni, Zhang Huan, Hu Chengxiao, Zhao Xiaohu
State Key Laboratory of Agricultural Microbiology, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Se-enriched Products Development and Quality Control, Ministry of Agriculture and Rural Affairs/ National-Local Joint Engineering Laboratory of Se-enriched Food Development, Ankang 725000, China.
J Exp Bot. 2024 Sep 27;75(18):5768-5789. doi: 10.1093/jxb/erae238.
Plants can recruit beneficial microbes to enhance their ability to resist disease. It is well established that selenium is beneficial in plant growth, but its role in mediating microbial disease resistance remains poorly understood. Here, we investigated the correlation between selenium, oilseed rape rhizosphere microbes, and Sclerotinia sclerotiorum. Soil application of 0.5 and 1.0 mg kg-1 selenium [selenate Na2SeO4, Se(VI) or selenite Na2SeO3, Se(IV)] significantly increased the resistance of oilseed rape to Sclerotinia sclerotiorum compared with no selenium application, with a disease inhibition rate higher than 20% in Se(VI)0.5, Se(IV)0.5 and Se(IV)1.0 mg kg-1 treatments. The disease resistance of oilseed rape was related to the presence of rhizosphere microorganisms and beneficial bacteria isolated from the rhizosphere inhibited Sclerotinia stem rot. Burkholderia cepacia and the synthetic community consisting of Bacillus altitudinis, Bacillus megaterium, Bacillus cereus, Bacillus subtilis, Bacillus velezensis, Burkholderia cepacia, and Flavobacterium anhui enhanced plant disease resistance through transcriptional regulation and activation of plant-induced systemic resistance. In addition, inoculation of isolated bacteria optimized the bacterial community structure of leaves and enriched beneficial microorganisms such as Bacillus, Pseudomonas, and Sphingomonas. Bacillus isolated from the leaves were sprayed on detached leaves, and it also performed a significant inhibition effect on Sclerotinia sclerotiorum. Overall, our results indicate that selenium improves plant rhizosphere microorganisms and increase resistance to Sclerotinia sclerotiorum in oilseed rape.
植物可以招募有益微生物来增强其抗病能力。硒对植物生长有益已得到充分证实，但其在介导微生物抗病性方面的作用仍知之甚少。在此，我们研究了硒、油菜根际微生物与核盘菌之间的相关性。与不施硒相比，土壤施用0.5和1.0 mg kg-1硒[硒酸钠Na2SeO4，Se(VI)或亚硒酸钠Na2SeO3，Se(IV)]显著提高了油菜对核盘菌的抗性，在Se(VI)0.5、Se(IV)0.5和Se(IV)1.0 mg kg-1处理中病害抑制率高于20%。油菜的抗病性与根际微生物的存在有关，从根际分离的有益细菌抑制了菌核病。洋葱伯克霍尔德菌以及由高地芽孢杆菌、巨大芽孢杆菌、蜡样芽孢杆菌、枯草芽孢杆菌、贝莱斯芽孢杆菌、洋葱伯克霍尔德菌和安徽黄杆菌组成的合成群落通过转录调控和激活植物诱导的系统抗性增强了植物抗病性。此外，接种分离出的细菌优化了叶片的细菌群落结构，并富集了芽孢杆菌、假单胞菌和鞘氨醇单胞菌等有益微生物。将从叶片中分离出的芽孢杆菌喷洒在离体叶片上，对核盘菌也有显著抑制作用。总体而言，我们的结果表明，硒改善了植物根际微生物，增强了油菜对核盘菌的抗性。
