研究各种肉瘤的循环肿瘤细胞的表面蛋白和抗体组合。

Investigating surface proteins and antibody combinations for detecting circulating tumor cells of various sarcomas.

机构信息

Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, PO Box 116250, Gainesville, FL, 32611, USA.

Department of Pediatrics, Division of Hematology-Oncology, University of Florida, Gainesville, FL, 32610, USA.

出版信息

Sci Rep. 2024 May 29;14(1):12374. doi: 10.1038/s41598-024-61651-w.

DOI:10.1038/s41598-024-61651-w

PMID:38811642

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11137101/

Abstract

Circulating tumor cells (CTCs) have gathered attention as a biomarker for carcinomas. However, CTCs in sarcomas have received little attention. In this work, we investigated cell surface proteins and antibody combinations for immunofluorescence detection of sarcoma CTCs. A microfluidic device that combines filtration and immunoaffinity using gangliosides 2 and cell surface vimentin (CSV) antibodies was employed to capture CTCs. For CTC detection, antibodies against cytokeratins 7 and 8 (CK), pan-cytokeratin (panCK), or a combination of panCK and CSV were used. Thirty-nine blood samples were collected from 21 patients of various sarcoma subtypes. In the independent samples study, samples were subjected to one of three antibody combination choices. Significant difference in CTC enumeration was found between CK and panCK + CSV, and between panCK and panCK + CSV. Upon stratification of CK samples, those of metastatic disease had a higher CTC number than those of localized disease. In the paired samples study involving cytokeratin-positive sarcoma subtypes, using panCK antibody detected more CTCs than CK. Similarly, for osteosarcoma, using panCK + CSV combination resulted in a higher CTC count than panCK. This study emphasized deliberate selection of cell surface proteins for sarcoma CTC detection and subtype stratification for studying cancers as heterogeneous as sarcomas.

摘要

循环肿瘤细胞 (CTC) 作为癌症的生物标志物受到了广泛关注。然而，肉瘤中的 CTC 却很少受到关注。在这项工作中，我们研究了细胞表面蛋白和抗体组合，以用于免疫荧光法检测肉瘤 CTC。我们使用一种微流控装置，该装置结合了使用神经节苷脂 2 和细胞表面波形蛋白 (CSV) 抗体的过滤和免疫亲和性，以捕获 CTC。为了检测 CTC，我们使用了针对细胞角蛋白 7 和 8 (CK)、泛细胞角蛋白 (panCK) 或 panCK 和 CSV 组合的抗体。从 21 名不同肉瘤亚型的患者中采集了 39 份血液样本。在独立样本研究中，样本接受了三种抗体组合选择之一。在 CTC 计数方面，CK 和 panCK+CSV 之间以及 panCK 和 panCK+CSV 之间存在显著差异。对 CK 样本进行分层后，转移性疾病的 CTC 数量高于局限性疾病。在涉及细胞角蛋白阳性肉瘤亚型的配对样本研究中，使用 panCK 抗体检测到的 CTC 比 CK 多。同样，对于骨肉瘤，使用 panCK+CSV 组合的 CTC 计数高于 panCK。这项研究强调了为肉瘤 CTC 检测和亚型分层选择细胞表面蛋白的重要性，以研究像肉瘤这样具有异质性的癌症。