清洁剂片对不同义齿基托材料上 S. mutans 和 C. Albicans 的抗菌活性。

Antimicrobial activity of cleanser tablets against S. mutans and C. Albicans on different denture base materials.

机构信息

Faculty of Dentistry, Department of Prosthodontics, Zonguldak Bulent Ecevit University, Kozlu, Zonguldak, Turkey.

Faculty of Medicine, Department of Microbiology, Zonguldak Bulent Ecevit University, Zonguldak, Turkey.

出版信息

BMC Oral Health. 2024 May 29;24(1):633. doi: 10.1186/s12903-024-04403-6.

DOI:10.1186/s12903-024-04403-6

PMID:38811903

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11134932/

Abstract

BACKGROUND

In this study, the antimicrobial activity of three different cleanser tablets on S. mutans and C. albicans adhesion to PMMA, polyamide and 3D printed resin was investigated.

METHODS

40 samples were prepared for PMMA (SR Triplex Hot), polyamide (Deflex) and 3D printed resin (PowerResins Denture) materials and divided into four subgroups for cleansers (Aktident™, Protefix™, Corega™ tablets and distilled water) (n = 5). After the surface preparations were completed, the samples were immersed separately in tubes containing the prepared microorganism suspension and incubated at 37˚C for 24 h. After the incubation, the samples were kept in the cleanser solutions. The samples were then transferred to sterile saline tubes. All the tubes were vortexed and 10 µl was taken from each of them. Sheep blood agar was inoculated for colony counting. The inoculated plates were incubated for 48 h for S. mutans and 24 h for C. albicans. After incubation, colonies observed on all plates were counted. Statistical analyses were done with three-way ANOVA and Tukey's multiple comparison test.

RESULTS

Polyamide material registered the highest colony count of S. mutans, whereas PMMA registered the lowest. Significant differences in S. mutans adherence (p = 0.002) were found between the three denture base materials, but no such difference in C. albicans adherence (p = 0.221) was identified between the specimens. All three cleanser tablets eliminated 98% of S. mutans from all the material groups. In all these groups, as well, the antifungal effect of Corega™ on C. albicans was significantly higher than those of the other two cleanser tablets.

CONCLUSIONS

According to the study's results, it may be better to pay attention to surface smoothness when using polyamide material to prevent microorganism retention. Cleanser tablets are clinically recommended to help maintain hygiene in removable denture users, especially Corega tablets that are more effective on C. albicans.

摘要

背景

本研究旨在探讨三种不同清洁剂片剂对 PMMA、聚酰胺和 3D 打印树脂上 S. mutans 和 C. albicans 黏附的抗菌活性。

方法

将 40 个样本制备为 PMMA（SR Triplex Hot）、聚酰胺（Deflex）和 3D 打印树脂（PowerResins Denture）材料，并分为清洁剂（Aktident™、Protefix™、Corega™片剂和蒸馏水）（n=5）的四个亚组。表面准备完成后，将样本分别浸入含有已制备微生物悬浮液的管中，并在 37°C 下孵育 24 小时。孵育后，将样本保存在清洁剂溶液中。然后将样本转移到无菌盐水中。所有管均涡旋，从每个管中取出 10µl。接种绵羊血琼脂进行菌落计数。将接种的平板孵育 48 小时用于 S. mutans，24 小时用于 C. albicans。孵育后，计数所有平板上观察到的菌落。使用三因素方差分析和 Tukey 多重比较检验进行统计分析。

结果

聚酰胺材料的 S. mutans 菌落计数最高，而 PMMA 最低。三种义齿基托材料之间 S. mutans 黏附的差异有统计学意义（p=0.002），但 C. albicans 黏附的差异无统计学意义（p=0.221）。三种清洁剂片剂均能从所有材料组中消除 98%的 S. mutans。在所有这些组中，Corega™对 C. albicans 的抗真菌作用明显高于其他两种清洁剂片剂。