Freitas-Fernandes Frederico Silva, Cavalcanti Yuri Wanderley, Ricomini Filho Antonio Pedro, Silva Wander José, Del Bel Cury Altair Antoninha, Bertolini Martinna Mendonça
Professor, Department of Dentistry, University Center of Maranhão, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil.
Graduate student, Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil.
J Prosthet Dent. 2014 Dec;112(6):1349-55. doi: 10.1016/j.prosdent.2014.07.004. Epub 2014 Sep 23.
Candida biofilms on denture surfaces are substantially reduced after a single immersion in denture cleanser. However, whether this effect is maintained when dentures are immersed in cleanser daily is unclear.
The purpose of this study was to evaluate the effect of the daily use of enzymatic cleanser on Candida albicans biofilms on denture base materials.
The surfaces of polyamide and poly(methyl methacrylate) resin specimens (n=54) were standardized and divided into 12 groups (n=9 per group), according to study factors (material type, treatment type, and periods of treatment). Candida albicans biofilms were allowed to form over 72 hours, after which the specimens were treated with enzymatic cleanser once daily for 1, 4, or 7 days. Thereafter, residual biofilm was ultrasonically removed and analyzed for viable cells (colony forming units/mm(2)) and enzymatic activity (phospholipase, aspartyl-protease, and hemolysin). Factors that interfered with the response variables were analyzed by 3-way ANOVA with the Holm-Sidak multiple comparison method (α=.05).
Polyamide resin presented more viable cells of Candida albicans (P<.001) for both the evaluated treatment types and periods. Although enzymatic cleansing significantly (P<.001) reduced viable cells, daily use did not maintain this reduction (P<.001). Phospholipase activity significantly increased with time (P<.001) for both materials and treatments. However, poly(methyl methacrylate) based resin (P<.001) and enzymatic cleansing treatment (P<.001) contributed to lower phospholipase activity. Aspartyl-protease and hemolysin activities were not influenced by study factors (P>.05).
Although daily use of an enzymatic cleanser reduced the number of viable cells and phospholipase activity, this treatment was not effective against residual biofilm over time.
义齿表面的念珠菌生物膜在单次浸泡于义齿清洁剂后会大幅减少。然而，当义齿每日浸泡于清洁剂中时，这种效果是否能持续尚不清楚。
本研究的目的是评估每日使用酶清洁剂对义齿基托材料上白色念珠菌生物膜的影响。
聚酰胺和聚甲基丙烯酸甲酯树脂标本（n = 54）的表面进行标准化处理，并根据研究因素（材料类型、处理类型和处理时间）分为12组（每组n = 9）。白色念珠菌生物膜在72小时内形成，之后标本每日用酶清洁剂处理1、4或7天。此后，通过超声去除残留生物膜，并分析活细胞（菌落形成单位/mm²）和酶活性（磷脂酶、天冬氨酸蛋白酶和溶血素）。采用三因素方差分析和Holm-Sidak多重比较法（α = 0.05）分析干扰响应变量的因素。
对于所评估的处理类型和时间，聚酰胺树脂上的白色念珠菌活细胞更多（P < 0.001）。尽管酶清洁显著减少了活细胞（P < 0.001），但每日使用并未维持这种减少（P < 0.001）。两种材料和处理方式下，磷脂酶活性均随时间显著增加（P < 0.001）。然而，聚甲基丙烯酸甲酯基树脂（P < 0.001）和酶清洁处理（P < 0.001）导致较低的磷脂酶活性。天冬氨酸蛋白酶和溶血素活性不受研究因素影响（P > 0.05）。
尽管每日使用酶清洁剂减少了活细胞数量和磷脂酶活性，但随着时间推移，这种处理对残留生物膜无效。
