Tai Bada-Lahu, Sun Tian-Xia, Dai Nayin-Tai, Yu Mu-Yao, Liu Qi, Huang Lu-Qi, Zheng Han
NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine (Mongolian Medicine), Mongolian Medical College, Inner Mongolia Minzu University Tongliao 028000, China State Key Laboratory for Quality Insurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Jilin Ginseng Academy, Changchun University of Chinese Medicine Changchun 130117, China.
Zhongguo Zhong Yao Za Zhi. 2024 May;49(9):2461-2467. doi: 10.19540/j.cnki.cjcmm.20240124.201.
In this study, the chemical components of ethanol extract from the aromatic parts of Syringa oblata were systematically separated and purified by silica gel column chromatography, thin layer plate preparation and liquid phase preparation. Combined with ultraviolet analyzer(UV), infrared analyzer(IR), nuclear magnetic resonance analyzer(NMR), high resolution mass spectrometer(HR-ESI-MS), X-ray diffraction and other spectrum technology as well as literature physicochemical data comparison methods for structural identification, a total of 10 compounds were identified. They were identified as oblatanoid D(1),(-)-T-muurolol(2), oblatanoid E-G(3-5), 14-noreudesma-3-hydroxy-3-en-2,9-dione(6), 1-isopropyl-2,7-dimethylnaphthalene(7), isocoradiol(8), α-calacorene(9), cadin-4-en-1-β-ol(10). Compound 1 is a new sesquiterpene compound that has not been reported, and the other 9 compounds are isolated from S. oblata for the first time. The compound 1 has a significant protective effect on the LPS-induced inflammatory injury model of RAW264.7 cells.
本研究采用硅胶柱色谱、制备薄层板及液相制备等方法，对紫丁香芳香部位乙醇提取物的化学成分进行了系统分离与纯化。结合紫外分析仪（UV）、红外分析仪（IR）、核磁共振分析仪（NMR）、高分辨质谱仪（HR-ESI-MS）、X射线衍射等光谱技术以及文献理化数据对比法进行结构鉴定，共鉴定出10个化合物。分别鉴定为紫丁香内酯D（1）、（-）-T-桉叶醇（2）、紫丁香内酯E-G（3-5）、14-去甲乌苏烷-3-羟基-3-烯-2,9-二酮（6）、1-异丙基-2,7-二甲基萘（7）、异柯伊利素（8）、α-卡拉烯（9）、杜松-4-烯-1-β-醇（10）。化合物1为未见报道的新倍半萜化合物，其余9个化合物均为首次从紫丁香中分离得到。化合物1对RAW264.7细胞LPS诱导的炎症损伤模型具有显著的保护作用。
