Rauf Abdur, Almasoud Najla, Ibrahim Muhammad, Alomar Taghrid S, Khalil Anees Ahmed, Khursheed Tara, Khan Muhammad Umer, Jan Muhammad Saeed, Bhardwaj Kanchan, Iriti Marcello, Sharma Rohit
Department of Chemistry, University of Swabi, 23561 Anbar, Khyber Pakhtunkhwa, Pakistan.
Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, 11671 Riyadh, Saudi Arabia.
Front Biosci (Landmark Ed). 2024 May 11;29(5):183. doi: 10.31083/j.fbl2905183.
The present study aimed to investigate the anti-diabetic, anti-cholinesterase, and anti-inflammatory potential of extracts from different parts of , including leaves, stem, and roots, as well as isolated column fractions (F-B-1 C, F-B-2 C, F-B-3 C, and F-B-4 C).
The extracts and subsequent fractions were evaluated for their inhibitory activity against key enzymes involved in diabetes [α-glucosidase and α-amylase], neurodegenerative diseases [acetylcholinesterase and butyrylcholinesterase], and inflammation (cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX)).
The results showed that leaf extract exhibited the highest α-glucosidase inhibitory activity (73.84%) and α-amylase inhibitory activity (76.29%) at 1000 µg/mL. The stem extract (65.50%) and F-B-2 C fraction (69.67%) also demonstrated significant α-glucosidase inhibitory activity. In terms of anti-cholinesterase activity, the extracts of roots, leaves, and stem showed promising inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with half maximal inhibitory concentration (IC50) values ranging from 50.50 to 474.83 µg/mL. The derived fractions (F-B-1 C, F-B-2 C, F-B-3 C, and F-B-4 C) also exhibited notable inhibition of AChE and BChE, with IC50 values from 91.85 to 337.94 µg/mL. Moreover, the F-B-3 C fraction demonstrated the highest COX-2 inhibitory potential (85.72%), followed by F-B-1 C (83.13%), the stem extract (80.85%), and the leaves extract (79.00%). The F-B-1 C fraction showed the highest 5-LOX inhibitory activity (87.63%), while the root extract exhibited the lowest inhibition (73.39%).
The results demonstrated promising bioactivity, suggesting the potential of as a source of natural compounds with therapeutic applications. Further studies are required to identify and isolate the active components responsible for these effects and to evaluate their efficacy and safety.
本研究旨在调查[植物名称]不同部位（包括叶、茎和根）的提取物以及分离得到的柱层析组分（F-B-1 C、F-B-2 C、F-B-3 C和F-B-4 C）的抗糖尿病、抗胆碱酯酶和抗炎潜力。
对提取物及其后续组分进行评估，检测它们对糖尿病相关关键酶[α-葡萄糖苷酶和α-淀粉酶]、神经退行性疾病相关酶[乙酰胆碱酯酶和丁酰胆碱酯酶]以及炎症相关酶（环氧合酶-2（COX-2）和5-脂氧合酶（5-LOX））的抑制活性。
结果显示，[植物名称]叶提取物在1000 µg/mL时表现出最高的α-葡萄糖苷酶抑制活性（73.84%）和α-淀粉酶抑制活性（76.29%）。茎提取物（65.50%）和F-B-2 C组分（69.67%）也表现出显著的α-葡萄糖苷酶抑制活性。在抗胆碱酯酶活性方面，根、叶和茎的提取物对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）显示出有前景的抑制作用，半数最大抑制浓度（IC50）值在50.50至474.83 µg/mL之间。衍生组分（F-B-1 C、F-B-2 C、F-B-3 C和F-B-4 C）对AChE和BChE也表现出显著抑制作用，IC50值在91.85至337.94 µg/mL之间。此外，F-B-3 C组分表现出最高的COX-2抑制潜力（85.72%），其次是F-B-1 C（83.13%）、茎提取物（80.85%）和叶提取物（79.00%）。F-B-1 C组分表现出最高的5-LOX抑制活性（87.63%），而根提取物的抑制活性最低（73.39%）。
结果表明[植物名称]具有有前景的生物活性，提示其作为具有治疗应用的天然化合物来源的潜力。需要进一步研究以鉴定和分离负责这些效应的活性成分，并评估它们的疗效和安全性。
