Abdel-Ghani Mohammed A, El-Sharawy Mohamed E, Zaid Ebtsam K, Shehabeldin Ahmed M, Dessouki Sherif M, Moustapha Moustapha E, Metwally Abd El-Salam M, El-Shamaa Ibrahim S
Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, AL-Ahsa, Saudi Arabia.
Department of Theriogenology, Faculty of Veterinary Medicine, Assuit University, Assuit, Egypt.
Reprod Domest Anim. 2024 May;59(5):e14613. doi: 10.1111/rda.14613.
Spermatozoa can experience negative changes when subjected to freezing and thawing, including lowered motility, viability and acrosome response. Herein, the effects of different concentrations of soybean lecithin nanoparticles on cryopreserved Holstein bull semen were examined. Semen was collected, cryopreserved and utilized for sperm kinetic parameter analysis following dilution, equilibration and thawing with 0.5% soybean lecithin (E1), the control extender, and 0.75% (E2), 0.5% (E3), 0.25% (E4) and 0.125% (E5) of lecithin nanoparticles. Results revealed that following dilution, the progressive motility (PM) at E3, E4 and E5 of lecithin nanoparticles was higher (p < .05) than it was for E2. After equilibration, compared to the E1, E2, and E3 values, the PM, vitality, normal morphology, membrane integrity and intact acrosome values at the E5 were consistently greater (p < .05). Comparing the percentages of intact acrosome and membrane integrity at E2 and E3 to E4 and E5, a substantial decrease (p < .05) was seen. Following thawing, the percentage of PM improved at E2 and E5, even though their mean PM values were similar (p > .05) compared to E1, E3 and E4. Vigour and progression parameters of sperm (DAP, DCL, DSL, VAP, VCL, VSL and STR) at E5 were higher (p < .05) than those at E1, E2, E3 and E4. In conclusion, the cryopreserved sperm from Holstein bulls revealed outstanding properties both after equilibration and after thawing with 0.125% lecithin nanoparticles, and they were sensitive to high dosages.
精子在冻融过程中会发生负面变化，包括活力降低、存活率下降和顶体反应。在此，研究了不同浓度的大豆卵磷脂纳米颗粒对荷斯坦公牛冷冻精液的影响。收集精液，进行冷冻保存，并在用0.5%大豆卵磷脂（E1，对照稀释液）、0.75%（E2）、0.5%（E3）、0.25%（E4）和0.125%（E5）的卵磷脂纳米颗粒进行稀释、平衡和解冻后，用于精子动力学参数分析。结果显示，稀释后，卵磷脂纳米颗粒E3、E4和E5组的前向运动率（PM）高于E2组（p < 0.05）。平衡后，与E1、E2和E3组相比，E5组的PM、活力、正常形态、膜完整性和完整顶体值始终更高（p < 0.05）。将E2和E3组的完整顶体和膜完整性百分比与E4和E5组进行比较，发现有显著下降（p < 0.05）。解冻后，E2和E5组的PM百分比有所提高，尽管它们的平均PM值与E1、E3和E4组相似（p > 0.05）。E5组精子的活力和运动参数（DAP、DCL、DSL 、VAP、VCL、VSL和STR）高于E1、E2、E3和E4组（p < 0.05）。总之，荷斯坦公牛的冷冻精子在平衡后以及用0.125%卵磷脂纳米颗粒解冻后均表现出优异的性能，并且它们对高剂量敏感。
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