Emamverdi M, Zhandi M, Zare Shahneh A, Sharafi M, Akbari-Sharif A
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Reprod Domest Anim. 2013 Dec;48(6):899-904. doi: 10.1111/rda.12183. Epub 2013 May 23.
The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin-based semen extender as a substitute for egg yolk-based extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different extenders: (i) Tris-based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT extenders. The results showed that the proportion of live post-thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT extenders. In addition, SL1, SL1.5 and SL2.5 extenders resulted in significantly lower percentage of early-apoptotic sperm than that in EYT extender. There were no significant differences in different semen extenders for percentage of post-thawed necrotic and late-apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between extenders. In conclusion, SL1.5 extender was better than other extenders in most in vitro evaluated sperm parameters.
本研究的目的是调查一种化学成分明确的大豆卵磷脂基精液稀释剂替代蛋黄基稀释剂用于公羊精液冷冻保存的效果。在本研究中，在繁殖季节从4只赞迪公羊采集了28份射精样本，然后将其合并在一起。合并后的精液被分成6等份，并用6种不同的稀释剂进行稀释：（i）含0.5%（w/v）大豆卵磷脂的Tris基稀释剂（TE，SL0.5），（ii）含1%（w/v）大豆卵磷脂的TE（SL1），（iii）含1.5%（w/v）大豆卵磷脂的TE（SL1.5），（iv）含2%（w/v）大豆卵磷脂的TE（SL2），（v）含2.5%（w/v）大豆卵磷脂的TE（SL2.5），以及（vi）含20%（v/v）蛋黄的TE（EYT）。解冻后，评估精子活力和运动参数、质膜和顶体完整性、凋亡状态和线粒体活性。结果表明，与其他精液稀释剂相比，SL1.5中的总活力和前向运动活力（分别为54.43±1.33%和25.43±0.96%）显著更高。与其他稀释剂相比，SL1.5中的精子运动参数（VAP、VSL、VCL、ALH和STR）显著更高，但SL1稀释剂除外。与其他精液稀释剂相比，SL1.5中的质膜完整性（48.86±1.38%）显著更高。此外，SL1.5稀释剂中顶体完整的精子百分比（85.35±2.19%）显著高于SL0.5、SL2.5和EYT稀释剂中的百分比。结果表明，与SL0.5、SL2和EYT稀释剂相比，SL1.5稀释剂中解冻后活精子的比例显著增加。此外，SL1、SL1.5和SL2.5稀释剂导致早期凋亡精子的百分比显著低于EYT稀释剂。不同精液稀释剂在解冻后坏死和晚期凋亡精子百分比方面没有显著差异。此外，结果表明，不同稀释剂之间具有活跃线粒体的活精子百分比存在细微差异。总之，在大多数体外评估的精子参数方面，SL1.5稀释剂优于其他稀释剂。
