Department of Obstetrics and Gynecology, Fırat University School of Medicine, Elazığ, Turkiye.
Department of Histology and Embryology, Fırat University School of Medicine, Elazığ, Turkiye.
Turk J Med Sci. 2023 Nov 25;53(6):1817-1824. doi: 10.55730/1300-0144.5752. eCollection 2023.
BACKGROUND/AIM: Surfactant is a surface-active substance that, in addition to its detergent effect, also has effects that reduce inflammation and fibrosis. Because of these effects, it was aimed herein to investigate the effect of intraperitoneal surfactant application on preventing postoperative peritoneal adhesion formation in a uterine horn adhesion model.
Twenty-one Wistar albino rats were randomly divided into 3 groups (G1-G3), as follows: G1 (n = 7): control group. The abdomen was opened and then closed; G2 (n = 7): adhesion group. The abdomen was opened. Then, a 2-cm linear incision was made over the right uterine horn, 2 mL of isotonic saline was administered intraperitoneally, and the abdomen was closed; and G3 (n = 7): treatment group. The abdomen was opened, a 2-cm linear incision was made over the right uterine horn, 2 mL (70 mg/kg) of surfactant was administered intraperitoneally, and the abdomen was closed. After 15 days, the rats were euthanized, the abdomens were reopened, and adhesion scoring was performed. After the right uterine horns were removed and fixed with 10% formalin, appropriate sections were taken from the traumatized tissue, stained with Masson's trichrome, and fibrosis and inflammation scoring were performed.
The adhesion area and intensity were significantly higher in G2 than in G1 and G3 (p = 0.001) and were similar in G1 and G3 (p = 0.165). While fibrosis and inflammation were significantly higher in G2 than in G1 and G3 (p = 0.001), there was no difference between G1 and G3 (p = 0.5).
Intraperitoneal surfactant administration at a dose of 70 mg/kg was found to be effective in preventing intraabdominal adhesion formation in a rat uterine horn model.
背景/目的：表面活性剂是一种具有表面活性的物质，除了具有去污作用外，还具有减轻炎症和纤维化的作用。由于这些作用，本研究旨在探讨腹腔内应用表面活性剂对预防子宫角粘连模型中术后腹膜粘连形成的影响。
将 21 只 Wistar 白化大鼠随机分为 3 组（G1-G3），如下：G1（n=7）：对照组。打开腹部，然后关闭；G2（n=7）：粘连组。打开腹部。然后，在右侧子宫角上做 2cm 的线性切口，向腹腔内注入 2mL 等渗盐水，关闭腹部；G3（n=7）：治疗组。打开腹部，在右侧子宫角上做 2cm 的线性切口，向腹腔内注入 2mL（70mg/kg）表面活性剂，关闭腹部。15 天后，处死大鼠，重新打开腹部，进行粘连评分。切除右侧子宫角，用 10%甲醛固定后，从受伤组织中取适当部位，用 Masson 三色染色，进行纤维化和炎症评分。
G2 的粘连面积和强度明显高于 G1 和 G3（p=0.001），G1 和 G3 之间相似（p=0.165）。G2 的纤维化和炎症明显高于 G1 和 G3（p=0.001），而 G1 和 G3 之间没有差异（p=0.5）。
以 70mg/kg 剂量腹腔内给予表面活性剂可有效预防大鼠子宫角模型中的腹腔内粘连形成。
