• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

鼠伤寒沙门氏菌用质粒DNA转化：粗糙型菌株与光滑型菌株之间的差异

Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.

作者信息

MacLachlan P R, Sanderson K E

出版信息

J Bacteriol. 1985 Jan;161(1):442-5. doi: 10.1128/jb.161.1.442-445.1985.

DOI:10.1128/jb.161.1.442-445.1985
PMID:3881397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214893/
Abstract

Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequencies 8- to 630-fold higher than with smooth strains or other rough mutants.

摘要

用氯化钙处理法将鼠伤寒沙门氏菌的脂多糖缺陷型突变体用质粒DNA进行转化。只有那些分别由于galE或rfaF突变而具有Rc或Rd2化学型的突变体，转化效率大于每微克DNA 10(5)个转化子，其频率比光滑菌株或其他粗糙突变体高8至630倍。

相似文献

1
Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.鼠伤寒沙门氏菌用质粒DNA转化：粗糙型菌株与光滑型菌株之间的差异
J Bacteriol. 1985 Jan;161(1):442-5. doi: 10.1128/jb.161.1.442-445.1985.
2
Influence of lipopolysaccharide and protein in the cell envelope on recipient capacity in conjugation of Salmonella typhimurium.鼠伤寒沙门氏菌细胞包膜中脂多糖和蛋白质对接合时受体能力的影响。
J Bacteriol. 1981 Oct;148(1):283-93. doi: 10.1128/jb.148.1.283-293.1981.
3
Electrotransformation in Salmonella typhimurium LT2.鼠伤寒沙门氏菌LT2中的电转化
Can J Microbiol. 1991 Jun;37(6):474-7. doi: 10.1139/m91-078.
4
High efficiency transformation of Salmonella typhimurium and Salmonella typhi by electroporation.通过电穿孔高效转化鼠伤寒沙门氏菌和伤寒沙门氏菌。
Mol Gen Genet. 1990 Aug;223(1):156-8. doi: 10.1007/BF00315809.
5
Effect of lipopolysaccharide mutations and temperature on plasmid transformation efficiency in Pseudomonas aeruginosa.
Can J Microbiol. 1986 May;32(5):436-8. doi: 10.1139/m86-082.
6
Resistance of gram-negative bacteria to purified bactericidal leukocyte proteins: relation to binding and bacterial lipopolysaccharide structure.革兰氏阴性菌对纯化杀菌白细胞蛋白的抗性：与结合及细菌脂多糖结构的关系。
J Clin Invest. 1980 Mar;65(3):619-28. doi: 10.1172/JCI109707.
7
Salmonella typhimurium strains carrying haemolysin plasmids and cloned haemolysin genes from Escherichia coli.携带溶血素质粒的鼠伤寒沙门氏菌菌株以及从大肠杆菌克隆的溶血素基因。
Ann Inst Pasteur Microbiol (1985). 1985 May-Jun;136A(3):289-301. doi: 10.1016/s0769-2609(85)80091-0.
8
The influence of cations on the permeability of the outer membrane of Salmonella typhimurium and other gram-negative bacteria.阳离子对鼠伤寒沙门氏菌及其他革兰氏阴性菌外膜通透性的影响。
Can J Microbiol. 1979 Apr;25(4):475-85. doi: 10.1139/m79-070.
9
Construction of defined galE mutants of Salmonella for use as vaccines.构建用于疫苗的沙门氏菌特定galE突变体。
J Infect Dis. 1987 Jul;156(1):167-74. doi: 10.1093/infdis/156.1.167.
10
Transfectability of rough strains of Salmonella typhimurium.鼠伤寒沙门氏菌粗糙菌株的转染能力。
J Bacteriol. 1975 Dec;124(3):1630-4. doi: 10.1128/jb.124.3.1630-1634.1975.

引用本文的文献

1
Effects of bcsE, luxS and rfaG genes on the physiology and pathogenicity of Salmonella Typhimurium.bcsE、luxS和rfaG基因对鼠伤寒沙门氏菌生理特性及致病性的影响。
Arch Microbiol. 2025 Feb 17;207(3):63. doi: 10.1007/s00203-025-04248-9.
2
Effective control of antibiotic resistance using a sonication-based combinational treatment and its application to fresh food.利用基于声处理的联合处理有效控制抗生素耐药性及其在新鲜食品中的应用。
Ultrason Sonochem. 2022 Nov;90:106198. doi: 10.1016/j.ultsonch.2022.106198. Epub 2022 Oct 10.
3
Conjugal DNA Transfer in Sodalis glossinidius, a Maternally Inherited Symbiont of Tsetse Flies.舌蝇共生菌索利斯中冈比亚亚种的交配 DNA 转移，一种采采蝇的母系遗传共生菌。
mSphere. 2020 Nov 4;5(6):e00864-20. doi: 10.1128/mSphere.00864-20.
4
Synchronized Electromechanical Shock Wave-Induced Bacterial Transformation.同步机电冲击波诱导的细菌转化
ACS Omega. 2019 May 15;4(5):8512-8521. doi: 10.1021/acsomega.9b00202. eCollection 2019 May 31.
5
Serotype-specific evolutionary patterns of antimicrobial-resistant Salmonella enterica.血清型特异性的抗微生物药物耐药性沙门氏菌的进化模式。
BMC Evol Biol. 2019 Jun 21;19(1):132. doi: 10.1186/s12862-019-1457-5.
6
Temperature-sensitive, lipopolysaccharide-deficient mutants of Salmonella typhimurium.鼠伤寒沙门氏菌温度敏感型、脂多糖缺陷型突变株。
World J Microbiol Biotechnol. 1994 Nov;10(6):681-5. doi: 10.1007/BF00327959.
7
Calcium chloride made E. coli competent for uptake of extraneous DNA through overproduction of OmpC protein.氯化钙通过过表达 OmpC 蛋白使大肠杆菌能够摄取外源 DNA。
Protein J. 2012 Jun;31(5):366-73. doi: 10.1007/s10930-012-9411-z.
8
Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni.脂寡糖和荚膜多糖在空肠弯曲菌抗微生物耐药性及自然转化中的作用
J Antimicrob Chemother. 2009 Mar;63(3):462-8. doi: 10.1093/jac/dkn529. Epub 2009 Jan 15.
9
Role of Gne and GalE in the virulence of Aeromonas hydrophila serotype O34.Gne和GalE在嗜水气单胞菌O34血清型毒力中的作用。
J Bacteriol. 2007 Jan;189(2):540-50. doi: 10.1128/JB.01260-06. Epub 2006 Nov 10.
10
Alteration of the rugose phenotype in waaG and ddhC mutants of Salmonella enterica serovar Typhimurium DT104 is associated with inverse production of curli and cellulose.肠炎沙门氏菌鼠伤寒血清型DT104的waaG和ddhC突变体中皱纹表型的改变与卷曲纤维和纤维素的反向产生有关。
Appl Environ Microbiol. 2006 Jul;72(7):5002-12. doi: 10.1128/AEM.02868-05.

本文引用的文献

1
Mapping of rfa Genes in Salmonella typhimurium by ES18 and P22 Transduction and by Conjugation.利用ES18和P22转导以及接合作用对鼠伤寒沙门氏菌中rfa基因进行定位
J Bacteriol. 1972 Oct;112(1):48-57. doi: 10.1128/jb.112.1.48-57.1972.
2
Formation of phage receptors induced by galactose in a galactose-sensitive mutant of Salmonella.半乳糖在沙门氏菌半乳糖敏感突变体中诱导噬菌体受体的形成。
Virology. 1960 Jun;11:508-10. doi: 10.1016/0042-6822(60)90093-3.
3
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
4
Influence of lipopolysaccharide and protein in the cell envelope on recipient capacity in conjugation of Salmonella typhimurium.鼠伤寒沙门氏菌细胞包膜中脂多糖和蛋白质对接合时受体能力的影响。
J Bacteriol. 1981 Oct;148(1):283-93. doi: 10.1128/jb.148.1.283-293.1981.
5
Regulation of the OmpA outer membrane protein of Escherichia coli.大肠杆菌外膜蛋白OmpA的调控
J Bacteriol. 1981 Sep;147(3):972-85. doi: 10.1128/jb.147.3.972-985.1981.
6
Genetic transformation.遗传转化
Annu Rev Biochem. 1981;50:41-68. doi: 10.1146/annurev.bi.50.070181.000353.
7
Role of recBC nuclease in Escherichia coli transformation.RecBC核酸酶在大肠杆菌转化中的作用。
J Bacteriol. 1980 Aug;143(2):1031-2. doi: 10.1128/jb.143.2.1031-1032.1980.
8
Transformation of Escherichia coli with plasmid deoxyribonucleic acid: calcium-induced binding of deoxyribonucleic acid to whole cells and to isolated membrane fractions.用质粒脱氧核糖核酸转化大肠杆菌：钙诱导的脱氧核糖核酸与完整细胞及分离的膜组分的结合
J Bacteriol. 1981 Feb;145(2):780-7. doi: 10.1128/jb.145.2.780-787.1981.
9
Rough mutants of Salmonella typhimurium: immunochemical and structural analysis of lipopolysaccharides from rfaH mutants.鼠伤寒沙门氏菌的粗糙突变体：rfaH突变体脂多糖的免疫化学和结构分析
J Gen Microbiol. 1980 Jan;116(1):25-32. doi: 10.1099/00221287-116-1-25.
10
Salmonella typhimurium mutants of RfaH-phenotype: genetics and antibiotic sensitivities.鼠伤寒沙门氏菌RfaH表型突变体：遗传学与抗生素敏感性
J Gen Microbiol. 1980 Jan;116(1):17-24. doi: 10.1099/00221287-116-1-17.