鼠伤寒沙门氏菌用质粒DNA转化：粗糙型菌株与光滑型菌株之间的差异
Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.
作者信息
MacLachlan P R, Sanderson K E
出版信息
J Bacteriol. 1985 Jan;161(1):442-5. doi: 10.1128/jb.161.1.442-445.1985.
Abstract
Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequencies 8- to 630-fold higher than with smooth strains or other rough mutants.
摘要
用氯化钙处理法将鼠伤寒沙门氏菌的脂多糖缺陷型突变体用质粒DNA进行转化。只有那些分别由于galE或rfaF突变而具有Rc或Rd2化学型的突变体，转化效率大于每微克DNA 10(5)个转化子，其频率比光滑菌株或其他粗糙突变体高8至630倍。
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