Hacker J, Hof H, Hughes C, Goebel W
Ann Inst Pasteur Microbiol (1985). 1985 May-Jun;136A(3):289-301. doi: 10.1016/s0769-2609(85)80091-0.
Like all other Salmonella typhimurium strains examined, the smooth variants SF1397 (LT2) and 1366 and also their semi-rough and rough derivatives are non-haemolytic. Nevertheless, two haemolysin (Hly) plasmids of E. coli belonging to the inc groups incFIII,IV (pSU316) and incI2 (pHly152) were able to be introduced into these strains by conjugation and stably maintained. A considerable percentage of the Hly+ transconjugants obtained had lost parts of their O-side chains, a result of selection for the better recipient capability of "semi-rough" variants rather than the direct influence of the Hly+ plasmids themselves. In contrast to the incFIII,IV plasmid pSU316, which exhibited higher conjugation rates with rough recipients, the incI2 plasmid pHly152 was accepted best by smooth strains. Transformation with cloned E. coli haemolysin (hly) determinant was inefficient (less than 10(-6)) for smooth strains, but 10(2) - 10(3) times higher for rough recipients, and was increased by the use of Salmonella-modified DNA. The transformants and transconjugants were relatively stable and showed the same haemolytic activity as the E. coli donor strains. The virulence of the Hly+ smooth, semi-rough and rough S. typhimurium strains was tested in two mouse models, and neither the mortality rate nor the ability to multiply within the mouse spleen was influenced by the hly determinants.
与所有其他检测过的鼠伤寒沙门氏菌菌株一样，光滑型变体SF1397（LT2）和1366及其半粗糙型和粗糙型衍生物均无溶血活性。然而，属于IncFIII、IV组（pSU316）和IncI2组（pHly152）的两种大肠杆菌溶血素（Hly）质粒能够通过接合作用导入这些菌株并稳定维持。获得的相当一部分Hly+转接合子失去了部分O侧链，这是选择“半粗糙”变体更好的受体能力的结果，而非Hly+质粒本身的直接影响。与粗糙型受体接合率较高的IncFIII、IV质粒pSU316不同，IncI2质粒pHly152最易被光滑型菌株接受。用克隆的大肠杆菌溶血素（hly）决定簇对光滑型菌株进行转化效率较低（低于10^(-6)），但对粗糙型受体的转化效率高10^2 - 10^3倍，并且使用经沙门氏菌修饰的DNA可提高转化效率。转化子和转接合子相对稳定，并且与大肠杆菌供体菌株表现出相同的溶血活性。在两种小鼠模型中测试了Hly+光滑型、半粗糙型和粗糙型鼠伤寒沙门氏菌菌株的毒力，hly决定簇对死亡率和在小鼠脾脏内繁殖的能力均无影响。
