School of Clinical Medicine, Xi'an Medical University, Xi'an, Shaanxi, 710021, PR China; Research Center for Prevention and Treatment of Respiratory Disease, Xi'an Medical University, Xi'an, Shaanxi, 710021, PR China.
School of Clinical Medicine, Xi'an Medical University, Xi'an, Shaanxi, 710021, PR China.
Biochem Biophys Res Commun. 2024 Sep 3;723:150159. doi: 10.1016/j.bbrc.2024.150159. Epub 2024 May 22.
Exercise has been recognized as an effective intervention in the treatment of pulmonary arterial hypertension (PAH), supported by numerous studies. However, the precise effects of exercise on pulmonary function remain to be fully elucidated. In this study, using a rat model of swimming exercise training and monocrotaline-induced PAH, we aimed to explore its impact on pulmonary morphology and function. Our investigations revealed that MCT-treated rats exhibited augmented mean pulmonary arterial pressure (MPAP) and pulmonary vascular remodeling, which can be attenuated by 4 weeks of swimming exercise training (60 min/day, 5 days/week). Notably, MCT-treated rats showed impaired pulmonary function, as manifested by decreased tidal volume and dynamic compliance, which were reversed by exercise training. Assessment of pulmonary substrate in PAH rats indicated a prominent pro-inflammatory substrate, evidenced by macrophage accumulation through quantitative immunohistological analysis of macrophage-like cell expression (CD68), and extracellular matrix remodeling, evaluated by Masson staining. Importantly, both the pro-inflammatory substrate and extracellular matrix remodeling were ameliorated by swimming exercise training. Additionally, serum biochemical analysis demonstrated elevated levels of low-density lipoprotein cholesterol and Apolipoprotein B following MCT treatment, which were reduced with exercise intervention. Moreover, exercise enhanced systemic insulin sensitivity in both MCT-treated and untreated rats. Notably, MCT and exercise treatment both decreased fasting blood glucose (FBG) levels in rats, whereas exercise training reinstated FBG levels to normal in MCT-treated rats. In summary, our study suggests that swimming exercise confers a pulmonary protective effect in MCT-induced PAH rats, highlighting the potential importance of exercise-based rehabilitation in the management of PAH.
运动已被公认为肺动脉高压（PAH）治疗的有效干预手段，这一观点得到了众多研究的支持。然而，运动对肺功能的确切影响仍有待充分阐明。在这项研究中，我们使用游泳运动训练和野百合碱诱导的 PAH 大鼠模型，旨在探讨其对肺形态和功能的影响。我们的研究表明，MCT 处理的大鼠表现出平均肺动脉压（MPAP）升高和肺血管重构，而 4 周的游泳运动训练（60 分钟/天，每周 5 天）可减轻这种情况。值得注意的是，MCT 处理的大鼠表现出肺功能受损，表现为潮气量和动态顺应性降低，而运动训练可逆转这种情况。对 PAH 大鼠的肺底物评估表明存在明显的促炎底物，这可通过定量免疫组织化学分析巨噬细胞样细胞表达（CD68）的巨噬细胞积累得到证实，并且通过 Masson 染色评估细胞外基质重构。重要的是，游泳运动训练可改善促炎底物和细胞外基质重构。此外，血清生化分析表明，MCT 处理后低密度脂蛋白胆固醇和载脂蛋白 B 水平升高，而运动干预可降低其水平。此外，运动增强了 MCT 处理和未处理大鼠的全身胰岛素敏感性。值得注意的是，MCT 和运动处理均降低了大鼠的空腹血糖（FBG）水平，而运动训练可使 MCT 处理的大鼠的 FBG 水平恢复正常。总之，我们的研究表明，游泳运动对 MCT 诱导的 PAH 大鼠具有肺保护作用，强调了运动康复在 PAH 管理中的重要性。
