Bailey L L, Jang J, Johnson W, Jolley W B
J Thorac Cardiovasc Surg. 1985 Feb;89(2):242-7.
Fourteen newborn (less than 7 days) goats were subjected to orthotopic cardiac transplantation with donor xenografts from size-matched lambs. Ten goats survived the operation (greater than 24 hours). Recipient animals received cyclosporine 48 and 24 hours before the operation and daily after the operation on a gradually reducing daily protocol. Recipients were also given pulse doses of methylprednisolone (100 mg/kg) and azathioprine (3 mg/kg) once a week, the dosage schedule being gradually reduced and azathioprine discontinued as recipients became long-term survivors (greater than 60 days). Seven recipients had radionuclide left ventriculography for measurement of left ventricular ejection fraction from 1 to 4 months postoperatively. Ejection fractions at 1 month in two recipients were 35% and 57%, and at 2 months the ejection fraction in one recipient was 58%. Serial ejection fractions from 1 to 4 months postoperatively in four recipients averaged 50%, 58%, 45%, and 45%. Survival in days among the 10 recipients was 24, 32, 44, 47, 60, 60, 78, 90, 120, and 165. Average survival was 72 days. There were no significant infections. Most animals showed mild-to-moderate subacute and chronic graft rejection at autopsy. One host showed no gross or microscopic graft rejection at autopsy on postoperative day 47. Tumor was not observed. These data suggest that long-term survival may be feasible for newborn recipients of cardiac xenografts with cyclosporine therapy and limited supplemental immunosuppression.
十四只新生（小于7日龄）山羊接受了原位心脏移植，供体为大小匹配的羔羊异种移植物。十只山羊术后存活（超过24小时）。受体动物在手术前48小时和24小时接受环孢素，并在术后每天按逐渐减量的方案给药。受体还每周接受一次大剂量甲泼尼龙（100mg/kg）和硫唑嘌呤（3mg/kg）静脉注射，随着受体成为长期存活者（超过60天），给药方案逐渐减量，硫唑嘌呤停药。七只受体在术后1至4个月进行放射性核素左心室造影以测量左心室射血分数。两名受体在术后1个月时的射血分数分别为35%和57%，一名受体在术后2个月时的射血分数为58%。四名受体术后1至4个月的连续射血分数平均为50%、58%、45%和45%。十名受体的存活天数分别为24、32、44、47、60、60、78、90、120和165天。平均存活天数为72天。无明显感染。大多数动物在尸检时表现为轻至中度亚急性和慢性移植物排斥反应。一只受体在术后第47天尸检时未发现明显或显微镜下的移植物排斥反应。未观察到肿瘤。这些数据表明，对于接受心脏异种移植的新生受体，采用环孢素治疗并辅以有限的免疫抑制，长期存活可能是可行的。
