Yu Xi, Li Shenglan, Ke Shuai, Ye Chenglin, Wang Qinghua, Wang Huaxin, Wang Lei
Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
Department of Radiography, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
J Cancer. 2024 Apr 19;15(11):3242-3253. doi: 10.7150/jca.94343. eCollection 2024.
Bladder Cancer (BCa) is one of the most common cancers of the urinary system. Colony-stimulating factor 2 (CSF2) is involved in many cancers, but not BCa. We investigated the effect of CSF2 on BCa in this study and the underlying molecular mechanisms. CSF2 mRNA levels in BCa were analyzed using the Cancer Genome Atlas (TCGA) database. Western blot was conducted to verify CSF2 expression in BCa tissue samples and cell lines. The effect of CSF2 on the growth of BCa cells was assessed by CCK8 and colony formation. To determine the migration and invasion capabilities of BCa cells, transwell analysis and wound healing assays were conducted. Next, western blot was used to explore the underlying mechanism. In the end, a xenografted BCa mouse model was established to examine the effects of CSF2 on tumorigenesis . Results showed that CSF2 mRNA was upregulated in BCa samples. Knocking down CSF2 significantly inhibited the proliferation and tumorigenesis of BCa cells and . Mechanism analysis revealed that CSF2 knockdown inhibited the proliferation and invasion of BCa cells via AKT/mTOR signaling. Based on these results, CSF2 promotes the proliferation and tumorigenesis of BCa.
膀胱癌（BCa）是泌尿系统最常见的癌症之一。集落刺激因子2（CSF2）与多种癌症有关，但与膀胱癌无关。在本研究中，我们调查了CSF2对膀胱癌的影响及其潜在的分子机制。使用癌症基因组图谱（TCGA）数据库分析膀胱癌中CSF2 mRNA水平。进行蛋白质免疫印迹法以验证CSF2在膀胱癌组织样本和细胞系中的表达。通过CCK8和集落形成评估CSF2对膀胱癌细胞生长的影响。为了确定膀胱癌细胞的迁移和侵袭能力，进行了Transwell分析和伤口愈合试验。接下来，使用蛋白质免疫印迹法探索潜在机制。最后，建立了异种移植膀胱癌小鼠模型以检查CSF2对肿瘤发生的影响。结果显示，CSF2 mRNA在膀胱癌样本中上调。敲低CSF2可显著抑制膀胱癌细胞的增殖和肿瘤发生。机制分析表明，敲低CSF2通过AKT/mTOR信号通路抑制膀胱癌细胞的增殖和侵袭。基于这些结果，CSF2促进膀胱癌的增殖和肿瘤发生。
