用于高分化、中分化和低分化肝细胞癌放射基因组学和放射蛋白质组学分析的LIRADS 5级图像引导下经福尔马林固定和冷冻组织芯穿刺活检的比较
Comparison of LIRADS 5 Image Guided Core Biopsy Derived From Formalin Fixed and Frozen Tissue Cores for Radiogenomics and Radioproteomics Analysis in Well, Moderate and Poorly Differentiated Hepatocellular Carcinoma.
作者信息
Simonian Margaret, Lu David S K, Whitelegge Julian, Cohn Whitaker, Ahuja Preeti, Hsu William, Raman Steven S
机构信息
Dept of Radiological Sciences, David Geffen School of Medicine, UCLA, USA.
Semel Institute for Neuroscience & Human Behavior, Pasarow mass spectrometry lab, David Geffen School of Medicine, UCLA, USA.
出版信息
Glob Med Ther. 2024;6. Epub 2024 Apr 22.
The aim of this pilot study is to evaluate and compare the quality of the genomics and proteomics data obtained from paired Formalin Fixed Paraffin Embedded (FFPE) and frozen (FF) tissue percutaneous core biopsies of Liver Imaging Reporting and Data System 5 (LIRADS 5) hepatocellular carcinoma (HCC) of varying histological grades. The preliminary data identified differentially expressed proteins and genes in poor, moderate and well differentiated HCC biopsies, with a greater efficacy in fresh frozen samples. The data offered valuable insights into the characteristics and suitability of samples for future studies.
这项初步研究的目的是评估和比较从不同组织学分级的肝脏影像报告和数据系统5（LIRADS 5）肝细胞癌（HCC）的配对福尔马林固定石蜡包埋（FFPE）和冷冻（FF）组织经皮核心活检中获得的基因组学和蛋白质组学数据的质量。初步数据确定了低分化、中分化和高分化HCC活检中差异表达的蛋白质和基因，新鲜冷冻样本的效果更佳。这些数据为未来研究样本的特征和适用性提供了有价值的见解。
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