Davis M J, Gore R W
Am J Physiol. 1985 Jan;248(1 Pt 2):H143-6. doi: 10.1152/ajpheart.1985.248.1.H143.
Existing methods of preparing the hamster cheek pouch for observation under an intravital microscope have several disadvantages. The everted method, described by Duling (Microvasc. Res. 5: 423-429, 1973), appears to restrict blood flow by placing unnatural tension on the retractor muscle and by requiring an incision in the tip of the pouch. The method of Yamaki et al. (Microvasc. Res. 21: 299-301, 1981) requires an incision in the tip of the pouch and complete disconnection of the retractor muscle. The chamber method of Greenblatt et al. (Microvasc. Res. 1: 420-432, 1969) has a limited optical resolution because the tissue cannot easily be transilluminated with properly condensed light. We have devised a less traumatic method of preparing the pouch, which eliminates these disadvantages. The hamster is anesthetized, and a thin, glass support plate is inserted into the left cheek pouch. The plate is constructed and positioned so that it does not restrict flow to any part of the pouch. The free end of the plate is secured to the animal stage. An incision is made in the skin to expose the cheek pouch, and the loose, avascular connective tissue investing the pouch and the retractor muscle is removed. The pouch is positioned at an angle of 20 degrees from the hamster's body in a temperature-controlled chamber over a standard microscope condensor system. Throughout both surgical and experimental procedures, the pouch is superfused with Ringer-bicarbonate solution at 37.5 degrees C. This preparation minimizes surgical trauma and allows the entire vascular supply to the cheek pouch to be studied.
现有的用于在活体显微镜下观察仓鼠颊囊的制备方法存在几个缺点。杜林（《微血管研究》5：423 - 429，1973年）描述的外翻法，似乎通过对牵开肌施加不自然的张力以及在颊囊尖端进行切口来限制血流。山木等人（《微血管研究》21：299 - 301，1981年）的方法需要在颊囊尖端进行切口并完全切断牵开肌。格林布拉特等人（《微血管研究》1：420 - 432，1969年）的腔室法具有有限的光学分辨率，因为组织难以用适当聚焦的光进行透照。我们设计了一种创伤较小的颊囊制备方法，该方法消除了这些缺点。将仓鼠麻醉，然后将一块薄的玻璃支撑板插入左侧颊囊。该板的构造和位置使其不会限制颊囊任何部位的血流。板的自由端固定在动物载物台上。在皮肤上做一个切口以暴露颊囊，并去除包裹颊囊和牵开肌的疏松、无血管结缔组织。将颊囊在温度控制室内以与仓鼠身体成20度角的方式放置在标准显微镜聚光系统上方。在整个手术和实验过程中，颊囊用37.5摄氏度的林格氏碳酸氢盐溶液进行灌注。这种制备方法将手术创伤降至最低，并允许对颊囊的整个血管供应进行研究。
