AO Research Institute Davos, Davos Platz, Switzerland.
Department of Oral and Maxillofacial Surgery, Tübingen University Hospital, Tübingen, Germany.
PLoS One. 2024 May 31;19(5):e0303035. doi: 10.1371/journal.pone.0303035. eCollection 2024.
Fracture non-unions affect many patients worldwide, however, known risk factors alone do not predict individual risk. The identification of novel biomarkers is crucial for early diagnosis and timely patient treatment. This study focused on the identification of microRNA (miRNA) related to the process of fracture healing. Serum of fracture patients and healthy volunteers was screened by RNA sequencing to identify differentially expressed miRNA at various times after injury. The results were correlated to miRNA in the conditioned medium of human bone marrow mesenchymal stromal cells (BMSCs) during in vitro osteogenic differentiation. hsa-miR-1246, hsa-miR-335-5p, and miR-193a-5p were identified both in vitro and in fracture patients and their functional role in direct BMSC osteogenic differentiation was assessed. The results showed no influence of the downregulation of the three miRNAs during in vitro osteogenesis. However, miR-1246 may be involved in cell proliferation and recruitment of progenitor cells. Further studies should be performed to assess the role of these miRNA in other processes relevant to fracture healing.
骨折不愈合影响全球许多患者，但已知的危险因素并不能预测个体风险。新型生物标志物的鉴定对于早期诊断和及时治疗患者至关重要。本研究专注于鉴定与骨折愈合过程相关的 microRNA（miRNA）。通过 RNA 测序筛选骨折患者和健康志愿者的血清，以鉴定损伤后不同时间点差异表达的 miRNA。将结果与体外成骨分化过程中人类骨髓间充质基质细胞（BMSC）条件培养基中的 miRNA 相关联。hsa-miR-1246、hsa-miR-335-5p 和 miR-193a-5p 在体外和骨折患者中均被鉴定出来，并评估了它们在直接 BMSC 成骨分化中的功能作用。结果表明，在体外成骨过程中这三种 miRNA 的下调没有影响。然而，miR-1246 可能参与细胞增殖和祖细胞的募集。应进一步开展研究以评估这些 miRNA 在与骨折愈合相关的其他过程中的作用。
