Potter Matthew L, Smith Kathryn, Vyavahare Sagar, Kumar Sandeep, Periyasamy-Thandavan Sudharsan, Hamrick Mark, Isales Carlos M, Hill William D, Fulzele Sadanand
Department of Orthopedics, Augusta University, Augusta, GA.
Department of Cell Biology and Anatomy, Augusta University, Augusta, GA.
Biomol Concepts. 2021 Oct 13;12(1):132-143. doi: 10.1515/bmc-2021-0015.
Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.
已知基质细胞衍生因子1（SDF-1）会影响骨髓基质细胞（BMSC）的迁移、成骨分化和骨折愈合。我们推测，SDF-1通过表观遗传调控，特别是通过微小RNA（miRNA）介导其对BMSC的一些作用。miRNA是靶向特定mRNA并阻止其翻译的小非编码RNA。我们进行了全局miRNA分析，并确定有几种miRNA在SDF-1处理后差异表达。基因表达综合数据库（GEO）数据集分析表明，这些miRNA在成骨分化和骨折愈合中起重要作用。KEGG和GO分析表明，SDF-1依赖性miRNA变化会影响多个细胞途径，包括脂肪酸生物合成、甲状腺激素信号传导和粘蛋白型O-聚糖生物合成途径。此外，生物信息学分析表明，有几种miRNA靶向与干细胞迁移和分化相关的基因。本研究结果表明，SDF-1通过miRNA调控诱导其对BMSC功能的一些作用。
