College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
Tianjin Huikang Biotechnology Co., LTD, Tianjin 300304, China.
Food Chem. 2024 Oct 1;454:139681. doi: 10.1016/j.foodchem.2024.139681. Epub 2024 May 22.
Oat protein isolate (OPI)/high methoxyl pectin (HMP) complexes (OPP) were prepared to stabilized Pickering emulsions and applied as nutraceutical delivery systems. The different mass ratios and pH changed the interactions between OPI and HMP that caused the different size of OPP. Specifically, smaller particle size of OPP (125.7-297.6 nm) were formed when hydrophobic interactions along with electrostatic forces predominant in OPP (OPI:HMP = 3:1, pH 4, 5). Among these particles, OPP-2 could stabilize Pickering emulsion efficiently through formation of dense interfacial film, which exhibited the highest apparent viscosity and the smallest average droplet size (23.39 μm). Moreover, OPP-2 stabilized Pickering emulsions with superior stability not only exhibited higher encapsulation efficiency of 85.63%, but also could control curcumin release in simulated gastrointestinal fluids to improve curcumin's bioaccessibility. These results verified the possibility of OPP to be a Pickering emulsions stabilizer, and also identified its potential to be a stable delivery system for bioactive compounds.
燕麦蛋白分离物(OPI)/高甲氧基果胶(HMP)复合物(OPP)被制备用于稳定 Pickering 乳液,并用作营养输送系统。不同的质量比和 pH 值改变了 OPI 和 HMP 之间的相互作用,导致 OPP 的大小不同。具体来说,当 OPP(OPI:HMP=3:1,pH4,5)中主要存在疏水相互作用和静电力时,形成了较小的 OPP 粒径(125.7-297.6nm)。在这些颗粒中,OPP-2 可以通过形成致密的界面膜有效地稳定 Pickering 乳液,其表现出最高的表观粘度和最小的平均液滴尺寸(23.39μm)。此外,OPP-2 稳定的 Pickering 乳液具有优异的稳定性,不仅表现出 85.63%的较高包封效率,而且可以控制姜黄素在模拟胃肠道液中的释放,从而提高姜黄素的生物利用度。这些结果验证了 OPP 作为 Pickering 乳液稳定剂的可能性,并确定了其作为生物活性化合物稳定输送系统的潜力。