Wang Qianqian, Wang Xinyu, Qi Xiaoxi, Liu Libo, Zhang Guofang, Du Peng, Li Chun
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China.
Heilongjiang Institute of Green Food Science, Northeast Agricultural University, Harbin 150028, China.
Foods. 2024 Nov 28;13(23):3854. doi: 10.3390/foods13233854.
Sanxan (SAN) is a novel microbial polysaccharide that is both safe and edible and represents a promising new source of food resources. It exhibits gelling properties and certain emulsifying properties. To date, there have been few studies published on the enhancement of protein emulsification by sanxan. In this study, three widely used proteins were used: casein (CS), pea protein isolate (PPI), and soy protein isolate (SPI). SAN-protein composite particles were prepared by non-covalent interactions to evaluate the availability of SAN in Pickering emulsions. The effect of SAN on the ability of the complexes to stabilize the emulsion was investigated by measuring and characterizing the physicochemical properties of three SAN-protein complexes. Fourier transform infrared (FTIR) and fluorescence spectroscopy analyses showed that SAN was able to bind to three proteins to form complexes. All three complexes formed by SAN with SPI, PPI and CS had good emulsification properties, with PPI-SAN being the best. Storage results showed better stability of the composite particle-stabilized emulsion. These results indicate that the complexation of SAN with proteins improves the emulsification of proteins and increases the stability of Pickering emulsions. The findings of this study provide valuable information for the utilization of SAN in emulsions.
桑 xan(SAN)是一种新型微生物多糖,既安全又可食用,是一种很有前景的新食品资源来源。它具有凝胶特性和一定的乳化特性。迄今为止,关于桑 xan 增强蛋白质乳化作用的研究报道很少。在本研究中,使用了三种广泛使用的蛋白质:酪蛋白(CS)、豌豆分离蛋白(PPI)和大豆分离蛋白(SPI)。通过非共价相互作用制备了 SAN - 蛋白质复合颗粒,以评估 SAN 在皮克林乳液中的可用性。通过测量和表征三种 SAN - 蛋白质复合物的物理化学性质,研究了 SAN 对复合物稳定乳液能力的影响。傅里叶变换红外(FTIR)和荧光光谱分析表明,SAN 能够与三种蛋白质结合形成复合物。SAN 与 SPI、PPI 和 CS 形成的所有三种复合物都具有良好的乳化性能,其中 PPI - SAN 最佳。储存结果表明复合颗粒稳定乳液具有更好的稳定性。这些结果表明,SAN 与蛋白质的络合改善了蛋白质的乳化作用,并提高了皮克林乳液的稳定性。本研究结果为桑 xan 在乳液中的利用提供了有价值的信息。