Roth W G, Leckie M P, Dietzler D N
Biochem Biophys Res Commun. 1985 Jan 16;126(1):434-41. doi: 10.1016/0006-291x(85)90624-2.
In intact Escherichia coli cells, severe osmotic stress almost totally inhibited active transport of carbohydrate by all of the systems known to transport carbohydrates in E. coli: group translocation (glucose), binding-protein mediated transport (maltose), proton symport (lactose), and sodium cotransport (melibiose). Detailed study of glucose transport showed that this inhibition of transport was not secondary to the inhibition of growth by osmotic stress, but rather that the inhibition of transport of a source of carbon and energy was sufficient to cause the complete inhibition of growth observed during severe osmotic upshock. Transport and growth inhibition did not result from cell death; upshocked cells were viable and metabolically active.
在完整的大肠杆菌细胞中，严重的渗透胁迫几乎完全抑制了大肠杆菌中所有已知的碳水化合物转运系统对碳水化合物的主动转运：基团转位（葡萄糖）、结合蛋白介导的转运（麦芽糖）、质子同向转运（乳糖）和钠协同转运（蜜二糖）。对葡萄糖转运的详细研究表明，这种转运抑制并非继发于渗透胁迫对生长的抑制，而是碳源和能源转运的抑制足以导致在严重渗透应激期间观察到的生长完全抑制。转运和生长抑制并非由细胞死亡引起；受到应激的细胞是有活力的且具有代谢活性。
