Belotskiĭ S M, Snastina T I, Dikovskaia E S
Biull Eksp Biol Med. 1985 Feb;99(2):174-6.
To detect Fc mu R, use was made of human and animal neutrophils. The detection of the receptors was possible only after the use of special manipulations: 1) cultivation for 18 h in a IgM-free of IgM-low medium, 2) treatment of neutrophils with hypotonic shock and then with acetate buffer solution. Both the methods provided similar results, permitting the detection on an average of 13-22% of neutrophils (up to 39%) carrying the receptors under consideration.
为检测FcμR,使用了人和动物的中性粒细胞。仅在采用特殊处理后才有可能检测到这些受体:1)在无IgM或低IgM培养基中培养18小时;2)用低渗休克处理中性粒细胞,然后用乙酸盐缓冲溶液处理。两种方法都得到了相似的结果,平均可检测到13% - 22%(最高可达39%)携带所研究受体的中性粒细胞。