Key Laboratory of Clinical Laboratory Diagnostics (Chinese Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Sichuan Nursing Vocational College,Chengdu 610100, People's Republic of China.
Anal Chem. 2024 Jun 11;96(23):9570-9575. doi: 10.1021/acs.analchem.4c01080. Epub 2024 Jun 1.
MicroRNA (miRNA) and apurinic/apyrimidinic endonuclease 1 (APE1) have been reported to be closely associated with cancers, making them potential crucial biomarkers and therapeutic targets. However, focusing on the detection of a single target is not conducive to the diagnosis and prognosis assessment of diseases. In this study, an AND logic-gate-based dual-locking hairpin-mediated catalytic hairpin assembly (DL-CHA) was developed for sensitive and specific detection of microRNA and APE1. By addition of a lock to each of the hairpins, with APE1 and microRNA serving as keys, fluorescence signals could only be detected in the presence of simultaneous stimulation by APE1 and miRNA-224. This indicated that the biosensor could operate as an AND logic gate. DL-CHA exhibited advantages such as a low background, rapid response, and high logic capability. Therefore, the biosensor serves as a novel approach to cancer diagnosis with significant potential applications.
MicroRNA (miRNA) 和脱嘌呤/脱嘧啶内切酶 1 (APE1) 已被报道与癌症密切相关，使它们成为潜在的关键生物标志物和治疗靶点。然而，专注于单个目标的检测不利于疾病的诊断和预后评估。在这项研究中，开发了一种基于与门的双锁发夹介导的催化发夹组装（DL-CHA），用于灵敏和特异性检测 microRNA 和 APE1。通过在每个发夹上添加一个锁，将 APE1 和 microRNA 作为钥匙，只有在同时受到 APE1 和 miRNA-224 的刺激时，才能检测到荧光信号。这表明该生物传感器可以作为与门逻辑工作。DL-CHA 具有背景低、响应快、逻辑能力强等优点。因此，该生物传感器是一种用于癌症诊断的新方法，具有重要的应用潜力。
