Mafune N, Ideta N, Watabe H, Nagura H, Kobayashi K
Clin Exp Immunol. 1985 Jan;59(1):123-31.
Rat liver arginase was isolated from extracts of liver in a pure form. Monospecific antisera raised against the arginase reacted with arginase of liver but not with arginase of kidney, spleen, heart, lung, testis and brain. The antisera were, however, reactive with liver arginase of a variety of animals, including human, mouse, sheep, horse and cow as well as rabbit, a homologous animal used for immunization. The rabbit autoantibodies showed direct cytotoxic activity as well as antibody-dependent cell-mediated cytotoxicity to homologous rabbit hepatocytes. Immunocytochemical electron microscopic examination showed that the arginase was localized on the surfaces of rat hepatocytes and on intracellular organelles. These results suggest that liver arginase could be an important antigen of the liver with implications for the pathogenesis of autoimmune liver diseases.
大鼠肝脏精氨酸酶以纯形式从肝脏提取物中分离出来。针对精氨酸酶产生的单特异性抗血清与肝脏中的精氨酸酶发生反应，但不与肾脏、脾脏、心脏、肺、睾丸和大脑中的精氨酸酶反应。然而，这些抗血清与多种动物的肝脏精氨酸酶有反应，包括人类、小鼠、绵羊、马和牛，以及用于免疫的同源动物兔子。兔自身抗体对同源兔肝细胞表现出直接细胞毒性活性以及抗体依赖性细胞介导的细胞毒性。免疫细胞化学电子显微镜检查表明，精氨酸酶定位于大鼠肝细胞表面和细胞内细胞器上。这些结果表明，肝脏精氨酸酶可能是肝脏的一种重要抗原，对自身免疫性肝病的发病机制具有重要意义。
