Yang Kun, Wang Ruyi, Lu Junjian, Wang Jin, Liao Xuewei, Wang Chen
Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China; Honors college, Nanjing Normal University, Nanjing, 210023, China.
Talanta. 2024 Sep 1;277:126331. doi: 10.1016/j.talanta.2024.126331. Epub 2024 May 30.
Recognition and separation of chiral isomers are of great importance in both industrial and biological applications. However, owing to identical molecular formulas and chemical properties of enantiomers, signal transduction and amplification are still two major challenges in chiral sensing. In this study, we developed an enantioselective device by integrating chiral covalent organic framework nanosheets (CONs) with nanochannels for sensitive identification and quantification of enantiomers. Using 3,4-dihydroxyphenylalanine (DOPA) as the model analyte, the as-prepared chiral nanofluidic device exhibits a remarkable chiral recognition ability to l-DOPA than d-DOPA. More importantly, due to the chelation of DOPA with Fe ions, it can efficiently block the ion transport through channel and shield the channel surface charge, which will amplify the difference in the electrochemical response of l-DOPA and d-DOPA. Therefore, a sensitive chiral recognition can be achieved using the present nanofluidic device coupled using electrochemical amplification strategy. Notably, using this method, an ultra-low concentration of l-DOPA (as low as 0.21 pM) can be facilely and successfully detected with a linear range of 1 pM-10 μM. This study provides a reliable and sensitive approach for achieving highly selective detection of chiral molecules.
手性异构体的识别与分离在工业和生物应用中都具有重要意义。然而，由于对映体具有相同的分子式和化学性质，信号转导与放大仍是手性传感领域的两大主要挑战。在本研究中，我们通过将手性共价有机框架纳米片（CONs）与纳米通道相结合，开发了一种对映体选择性装置，用于对映体的灵敏识别与定量分析。以3,4 - 二羟基苯丙氨酸（DOPA）作为模型分析物，所制备的手性纳米流体装置对l - DOPA表现出比d - DOPA更强的手性识别能力。更重要的是，由于DOPA与铁离子的螯合作用，它能够有效阻断离子通过通道的传输，并屏蔽通道表面电荷，这将放大l - DOPA和d - DOPA在电化学响应上的差异。因此，利用当前的纳米流体装置结合电化学放大策略可实现灵敏的手性识别。值得注意的是，采用这种方法，能够轻松且成功地检测到超低浓度的l - DOPA（低至0.21 pM），线性范围为1 pM - 10 μM。本研究为实现手性分子的高选择性检测提供了一种可靠且灵敏的方法。
