Zhang Zhong-Hong, Zheng Jian-Wei, Liu Si-Fen, Hao Ting-Bin, Yang Wei-Dong, Li Hong-Ye, Wang Xiang
Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
College of Food Science and Engineering, Foshan University, Foshan 528231, China.
Environ Res. 2024 Sep 15;257:119291. doi: 10.1016/j.envres.2024.119291. Epub 2024 May 30.
The presence of butylparaben (BP), a prevalent pharmaceutical and personal care product, in surface waters has raised concerns regarding its impact on aquatic ecosystems. Despite its frequent detection, the toxicity of BP to the cyanobacterium Microcystis aeruginosa remains poorly understood. This study investigates the influence of BP on the growth and physiological responses of M. aeruginosa. Results indicate that low concentrations of BP (below 2.5 mg/L) have negligible effects on M. aeruginosa growth, whereas higher concentrations (5 mg/L and 10 mg/L) lead to significant growth inhibition. This inhibition is attributed to the severe disruption of photosynthesis, evidenced by decreased Fv/Fm values and chlorophyll a content. BP exposure also triggers the production of reactive oxygen species (ROS), resulting in elevated activity of antioxidant enzymes. Excessive ROS generation stimulates the production of microcystin-LR (MC-LR). Furthermore, lipid peroxidation and cell membrane damage indicate that high BP concentrations cause cell membrane rupture, facilitating the release of MC-LR into the environment. Transcriptome analysis reveals that BP disrupts energy metabolic processes, particularly affecting genes associated with photosynthesis, carbon fixation, electron transport, glycolysis, and the tricarboxylic acid cycle. These findings underscore the profound physiological impact of BP on M. aeruginosa and highlight its role in stimulating the production and release of MC-LR, thereby amplifying environmental risks in aquatic systems.
对羟基苯甲酸丁酯（BP）作为一种常见的药品和个人护理产品，其在地表水中的存在引发了人们对其对水生生态系统影响的担忧。尽管BP经常被检测到，但其对铜绿微囊藻的毒性仍知之甚少。本研究调查了BP对铜绿微囊藻生长和生理反应的影响。结果表明，低浓度的BP（低于2.5毫克/升）对铜绿微囊藻的生长影响可忽略不计，而较高浓度（5毫克/升和10毫克/升）则导致显著的生长抑制。这种抑制归因于光合作用的严重破坏，Fv/Fm值和叶绿素a含量降低证明了这一点。BP暴露还会引发活性氧（ROS）的产生，导致抗氧化酶活性升高。过量的ROS生成刺激了微囊藻毒素-LR（MC-LR）的产生。此外，脂质过氧化和细胞膜损伤表明，高浓度的BP会导致细胞膜破裂，促使MC-LR释放到环境中。转录组分析表明，BP扰乱了能量代谢过程，尤其影响与光合作用、碳固定、电子传递、糖酵解和三羧酸循环相关的基因。这些发现强调了BP对铜绿微囊藻具有深远的生理影响，并突出了其在刺激MC-LR产生和释放方面的作用，从而加剧了水生系统中的环境风险。
