Proliferating and migrating effects of regenerating sea anemone cells-derived exosomes on human skin fibroblasts.

Regenerative effects of sea anemone-derived exosomes on human foreskin fibroblasts (HFFs) were investigated. Water-based extracts from regenerating tissue were collected at various time points, and exosomes were extracted after inducing wounds. Both the extract and exosomes were tested on HFF for proliferation and wound healing. analysis explored protein-protein docking between regenerative exosome proteins and HFF receptors. The MTT (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyltetrazolium bromide proliferation assay and wound healing test of aquatic extract showed proliferative effects on HFF cell lines, with the 60 μg/mL concentration significantly enhancing cell migration. Exosomes were characterised. Exosomes showed a significantly positive effect on cell proliferation and migration at the 50 µg/mL concentration 48 h post-wound induction. analysis revealed potential binding affinities between exosome proteins and HFF receptors. In conclusion, optimised concentrations of -derived exosomes exhibited positive effects on HFF regeneration and migration, suggesting their potential in accelerating wound healing.