Dobrzycka-Krahel Aldona, Steć Aleksandra, Czyrski Grzegorz S, Heinz Andrea, Dziomba Szymon
Business Faculty, WSB Merito University in Gdansk, Al. Grunwaldzka 238 A, 80-266 Gdansk, Poland.
Department of Toxicology, Faculty of Pharmacy, Medical University of Gdansk, 107 Hallera Street, 80-416 Gdansk, Poland.
Biology (Basel). 2025 Jul 23;14(8):922. doi: 10.3390/biology14080922.
A moon jellyfish () is a representative of the phylum Cnidaria, commonly found in the northern seas of the globe. The regenerative abilities of cnidarians have recently been associated with extracellular vesicles (EVs) secreted by these organisms. In this study, a method for the isolation of EVs from the oral arms of is presented. The methodology includes differential centrifugation, size-exclusion chromatography, and ultrafiltration. The isolates were characterized with tunable resistive pulse sensing, cryogenic transmission electron microscopy, capillary electrophoresis (CE), and electrophoretic light scattering (ELS). Small (<150 nm in diameter) EVs were abundant in the isolates. The EVs were found to carry nucleic acids, indicating their role in signaling. Additionally, the difference in zeta potential values measured with ELS and CE indicates high glycation in the vesicles analyzed. Although the method developed was effective in isolating EVs from small sample volumes (0.5 mL), the EV yield was insufficient for omics analysis. Thus, the scaling up of the isolation process is required for comprehensive biochemical analysis and biological activity assessment in -derived EVs.
海月水母是刺胞动物门的代表物种，常见于全球的北海域。刺胞动物的再生能力最近与这些生物体分泌的细胞外囊泡（EVs）相关联。在本研究中，提出了一种从海月水母口腕中分离EVs的方法。该方法包括差速离心、尺寸排阻色谱和超滤。通过可调电阻脉冲传感、低温透射电子显微镜、毛细管电泳（CE）和电泳光散射（ELS）对分离物进行表征。分离物中富含小（直径<150 nm）EVs。发现这些EVs携带核酸，表明它们在信号传导中的作用。此外，用ELS和CE测量的zeta电位值的差异表明所分析的囊泡中存在高度糖基化。尽管所开发的方法在从小体积样品（0.5 mL）中分离EVs方面是有效的，但EV产量不足以进行组学分析。因此，需要扩大分离过程以对海月水母来源的EVs进行全面的生化分析和生物活性评估。
