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整合蛋白质组学、转录组学和代谢组学为研究草地早熟禾耐镉和积累镉的机制提供了新的见解。

Integrated proteomics, transcriptomics, and metabolomics offer novel insights into Cd resistance and accumulation in Poa pratensis.

机构信息

College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-US. Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu 730070, China.

College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-US. Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu 730070, China.

出版信息

J Hazard Mater. 2024 Aug 5;474:134727. doi: 10.1016/j.jhazmat.2024.134727. Epub 2024 May 24.

Abstract

Kentucky bluegrass (Poa pratensis L., KB) demonstrates superior performance in both cadmium (Cd) accumulation and tolerance; however, the regulatory mechanisms and detoxification pathways in this species remain unclear. Therefore, phenotype, root ultrastructure, cell wall components, proteomics, transcriptomics, and metabolomics were analyzed under the hydroponic system to investigate the Cd tolerance and accumulation mechanisms in the Cd-tolerant KB variety 'Midnight (M)' and the Cd-sensitive variety 'Rugby II (R)' under Cd stress. The M variety exhibited higher levels of hydroxyl and carboxyl groups as revealed by Fourier transform infrared spectroscopy spectral analysis. Additionally, a reduced abundance of polysaccharide degradation proteins was observed in the M variety. The higher abundance of glutathione S-transferase and content of L-cysteine-glutathione disulfide and oxidized glutathione in the M variety may contribute to better performance of the M variety under Cd stress. Additionally, the R variety had an enhanced content of carboxylic acids and derivatives, increasing the Cd translocation capacity. Collectively, the down-regulation of cell wall polysaccharide degradation genes coupled with the up-regulation of glutathione metabolism genes enhances the tolerance to Cd stress in KB. Additionally, lignification of the endodermis and the increase in carboxylic acids and derivatives play crucial roles in the redistribution of Cd in KB.

摘要

肯塔基蓝草(Poa pratensis L.,KB)在镉(Cd)积累和耐受方面表现出优异的性能;然而,该物种的调节机制和解毒途径仍不清楚。因此,在水培系统下分析了表型、根超微结构、细胞壁成分、蛋白质组学、转录组学和代谢组学,以研究镉耐受品种“Midnight(M)”和镉敏感品种“Rugby II(R)”在镉胁迫下的镉耐受和积累机制。傅里叶变换红外光谱分析表明,M 品种的羟基和羧基含量较高。此外,M 品种中多糖降解蛋白的丰度降低。M 品种中谷胱甘肽 S-转移酶和 L-半胱氨酸-谷胱甘肽二硫醚和氧化谷胱甘肽的含量较高,可能有助于 M 品种在 Cd 胁迫下更好地发挥作用。此外,R 品种中羧酸及其衍生物的含量增加,增加了 Cd 的转运能力。总之,细胞壁多糖降解基因的下调与谷胱甘肽代谢基因的上调相结合,增强了 KB 对 Cd 胁迫的耐受性。此外,内皮层的木质化以及羧酸及其衍生物的增加在 KB 中 Cd 的再分配中起着关键作用。

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