Du Hanmei, Tan Lu, Wei Changhe, Li Shengchun, Xu Zhou, Wang Qinghai, Yu Qiuzhu, Ryan Peter R, Li Hongyou, Wang An'hu
Panxi Featured Crops Research and Utilization Key Laboratory of Sichuan Province, Xichang University, No. 1 Xuefu Road, An'ning, Xichang, 615000, People's Republic of China.
Division of Plant Sciences, Research School of Biology, The Australian National University, Canberra, ACT, 2601, Australia.
Sci Rep. 2025 Feb 11;15(1):5100. doi: 10.1038/s41598-025-89462-7.
Cadmium (Cd) can adversely damage plant growth. Therefore, understanding the control molecular mechanisms of Cd accumulation will benefit the development of strategies to reduce Cd accumulation in plants. This study performed transcriptomic and metabolomic analyses on the roots of a Cd-tolerant Tartary buckwheat cultivar following 0 h (CK), 6 h (T1), and 48 h (T2) of Cd treatment. The fresh weight and root length were not significantly inhibited under the T1 treatment but they were in the T2 treatment. The root's ultrastructure was seriously damaged in T2 but not in T1 treatment. This was evidenced by deformed cell walls, altered shape and number of organelles. A total of 449, 999 differentially expressed genes (DEGs) and eight, 37 differentially expressed metabolites (DEMs) were identified in the CK versus T1 and CK versus T2 comparison, respectively. DEGs analysis found that the expression of genes related to cell wall function, glutathione (GSH) metabolism, and phenylpropanoid biosynthesis changed significantly during Cd stress. Several WRKY, MYB, ERF, and bHLH transcription factors and transporters also responded to Cd treatment. Our results indicate that Cd stress affects cell wall function and GSH metabolism and that changes in these pathways might contribute to mechanisms of Cd tolerance in Tartary buckwheat.
镉（Cd）会对植物生长产生不利影响。因此，了解镉积累的控制分子机制将有助于制定减少植物中镉积累的策略。本研究对耐镉苦荞品种的根系在镉处理0小时（CK）、6小时（T1）和48小时（T2）后进行了转录组和代谢组分析。在T1处理下，鲜重和根长没有受到显著抑制，但在T2处理下受到了抑制。在T2处理中，根的超微结构受到严重破坏，而在T1处理中没有。这通过细胞壁变形、细胞器形状和数量的改变得到了证明。在CK与T1以及CK与T2的比较中，分别鉴定出449个、999个差异表达基因（DEG）和8个、37个差异表达代谢物（DEM）。DEG分析发现，在镉胁迫期间，与细胞壁功能、谷胱甘肽（GSH）代谢和苯丙烷生物合成相关的基因表达发生了显著变化。一些WRKY、MYB、ERF和bHLH转录因子以及转运蛋白也对镉处理有反应。我们的结果表明，镉胁迫影响细胞壁功能和GSH代谢，这些途径的变化可能有助于苦荞对镉的耐受机制。
