Clark D P
J Bacteriol. 1985 Mar;161(3):1049-53. doi: 10.1128/jb.161.3.1049-1053.1985.
A mutant of Escherichia coli (mdoR) has been isolated which is defective in synthesis of the membrane-derived oligosaccharides (MDO) normally found in the periplasmic space. In media of high osmotic pressure this defect is suppressed and MDO levels approaching those of the wild type are produced. The mdoR mutant also fails to accumulate glycogen; however, genetic analysis showed that mdoR was not cotransducible with the known glg (glycogen) locus. A further relationship between MDO and glycogen metabolism was suggested by two observations that (i) certain glg mutants affect MDO accumulation and (ii) elevated osmotic pressure inhibits glycogen accumulation, in both wild-type and mdoR cells.
已分离出一种大肠杆菌突变体(mdoR),它在合成通常存在于周质空间的膜衍生寡糖(MDO)方面存在缺陷。在高渗透压培养基中,这种缺陷会被抑制,产生的MDO水平接近野生型。mdoR突变体也无法积累糖原;然而,遗传分析表明,mdoR与已知的glg(糖原)基因座不能共转导。通过两项观察结果提示了MDO与糖原代谢之间的进一步关系:(i)某些glg突变体影响MDO积累;(ii)在野生型和mdoR细胞中,渗透压升高均抑制糖原积累。