Cell processes in dentin tubules during early phases of attachment to demineralized periodontitis-affected surfaces.

This report details some preliminary observations regarding cell morphology and behavior during early phases of in vivo attachment to demineralized surfaces. Rectangular dentin specimens prepared from beneath calculus-covered areas of root surfaces were treated with citric acid and implanted vertically into the skin of rats such that one end of the implant protruded above the skin. The implants were removed after 1 day and the dentin surface - connective tissue interface examined using scanning electron microscopy. A dynamic series of biological events seemed to be in progress. Numerous cells were attached to the dentinal root surface. Cell processes extended a considerable distance along the root surface and into dentin tubules. Cell processes in dentin tubules are discussed.