Li C, Calabrese R L
J Comp Neurol. 1985 Feb 15;232(3):414-24. doi: 10.1002/cne.902320312.
The distribution of proctolin-like immunoreactive (PLI) cells was mapped in the central nervous system of the leech Hirudo medicinalis. In segmental ganglia, PLI cells can be divided into two groups: cells that stain repeatedly in every successive ganglion, and cells that stain only in specific segmental ganglia. The number of PLI cells, therefore, ranged from eight to 20 cells per ganglion. One bilateral pair of PLI cells (cells PLI-1) was further characterized morphologically by Lucifer yellow and horseradish peroxidase cell injections. Cell PLI-1 conforms in soma position, morphology, and physiological properties with cell 101, which has been previously classified as an inhibitory motoneuron to the flattener muscles. A locust bioassay (O'Shea and Adams, Science 213:567-569, 1981) was used to detect the presence of proctolin-like bioactivity. Extracts of leech ganglia when applied to the extensor tibialis muscle of the locust leg induced a proctolin-like response similar to the responses induced by proctolin standards. This work extends the finding of proctolin-like substances to the annelid phylum.
对医用水蛭中枢神经系统中类促肠肌肽免疫反应性（PLI）细胞的分布进行了定位。在节段神经节中，PLI细胞可分为两组：在每个连续神经节中反复染色的细胞，以及仅在特定节段神经节中染色的细胞。因此，每个神经节中PLI细胞的数量在8到20个之间。通过荧光黄和辣根过氧化物酶细胞注射对一对双侧PLI细胞（PLI-1细胞）进行了进一步的形态学特征分析。PLI-1细胞在胞体位置、形态和生理特性上与101细胞相符，101细胞先前已被归类为扁平肌的抑制性运动神经元。采用蝗虫生物测定法（奥谢和亚当斯，《科学》213:567 - 569，1981）检测类促肠肌肽生物活性的存在。将水蛭神经节提取物应用于蝗虫腿部的胫伸肌时，会诱导出类似于促肠肌肽标准品诱导的类促肠肌肽反应。这项工作将类促肠肌肽物质的发现扩展到了环节动物门。
