Baccarini M, Bistoni F, Lohmann-Matthes M L
J Immunol. 1985 Apr;134(4):2658-65.
Bone marrow cells, cultured in L-929 CSF, consist of cells of granulocyte and macrophage lineages. Cells of the granulocyte lineage are known to be cytotoxic for Candida albicans. In this paper we report that macrophage precursor cells also display strong cell-mediated cytotoxicity against the yeast form of the dimorphic fungus C. albicans. The macrophage precursors responsible for this activity are nylon wool-nonadherent, nonphagocytic cells and lack asialo GM1 surface antigen. A purified population of macrophage precursors (greater than 95%) was obtained by means of Percoll density centrifugation. The interaction of these purified effectors with the target yeast cells was analyzed at a single cell level, and their activity was compared with that displayed by cells of the granulocytic series derived from the same bone marrow culture. Macrophage precursor cells proved to be more effective in binding the target cells and showed the same killing ability as the granulocytes: macrophage precursors were not damaged by contact with the target, in contrast to that which happened with granulocytes. In a long-term colony-forming unit assay, in fact, granulocytic cells showed a decrease over time in their ability to inhibit the growth of C. albicans, probably due to cell damage and death after the interaction with the target. In contrast, no loss of activity was observed with the macrophage precursor fraction. The same macrophage precursor cells also proved able to exert good natural killer activity against YAC-1 lymphoma cells, but not against P815 mastocytoma cells, as reported previously. The macrophage precursor cells, when cultivated in vitro to mature macrophages, lost completely their natural cytotoxicity against C. albicans and YAC-1 cells. The implications of these findings, as well as the possible role in vivo of such a precursor cell population during an infection, are discussed.
在L-929集落刺激因子中培养的骨髓细胞，由粒细胞系和巨噬细胞系的细胞组成。已知粒细胞系的细胞对白色念珠菌具有细胞毒性。在本文中，我们报告巨噬细胞前体细胞对双态真菌白色念珠菌的酵母形式也表现出强烈的细胞介导的细胞毒性。负责这种活性的巨噬细胞前体是尼龙毛非黏附性、非吞噬性细胞，并且缺乏唾液酸GM1表面抗原。通过Percoll密度离心获得了纯化的巨噬细胞前体群体（大于95%）。在单细胞水平分析了这些纯化的效应细胞与靶酵母细胞的相互作用，并将它们的活性与来自同一骨髓培养物的粒细胞系细胞所表现出的活性进行了比较。事实证明，巨噬细胞前体细胞在结合靶细胞方面更有效，并且与粒细胞表现出相同的杀伤能力：与粒细胞不同，巨噬细胞前体与靶细胞接触后不会受损。实际上，在长期集落形成单位试验中，粒细胞抑制白色念珠菌生长的能力随时间下降，这可能是由于与靶细胞相互作用后细胞受损和死亡。相比之下，巨噬细胞前体部分未观察到活性丧失。同样的巨噬细胞前体细胞也被证明能够对YAC-1淋巴瘤细胞发挥良好的自然杀伤活性，但对P815肥大细胞瘤细胞则没有，如先前报道的那样。当巨噬细胞前体细胞在体外培养成成熟巨噬细胞时，它们对白色念珠菌和YAC-1细胞的天然细胞毒性完全丧失。讨论了这些发现的意义，以及这种前体细胞群体在感染期间体内可能发挥的作用。
