在颗粒计数免疫测定中抑制独特型-抗独特型反应作为IgE的初步检测方法。

Inhibition of idiotype-anti-idiotype reaction in particle counting immunoassay as a tentative assay of IgE.

作者信息

Sluis K, Magnusson C G, Masson P L

出版信息

J Immunol Methods. 1985 Feb 11;76(2):255-61. doi: 10.1016/0022-1759(85)90302-3.

Abstract

Rabbit antibodies were raised against a purified mouse monoclonal IgG1 antibody having specificity for the D epsilon 2 determinant of human IgE. After appropriate absorption this antiserum was anti-idiotype specific and was used to set up a particle counting immunoassay for human IgE. Latex particles coated with F(ab')2 fragments of the anti-idiotypic antibodies were agglutinated by the monoclonal anti-IgE antibody (20 ng/ml). This reaction was inhibited up to 100% by human IgE and the assay took 30 min to perform with a sensitivity of 40 IU/ml. The coefficient of correlation with a routine IgE assay was r = 0.96, and the mean analytical recovery tested on 10 sera was 95.8%.

摘要

用对人IgE的Dε2决定簇具有特异性的纯化小鼠单克隆IgG1抗体免疫家兔，制备兔抗体。经过适当吸收后，该抗血清具有抗独特型特异性，并用于建立人IgE的粒子计数免疫测定法。用抗独特型抗体的F(ab')2片段包被的乳胶颗粒可被单克隆抗IgE抗体（20 ng/ml）凝集。该反应可被人IgE抑制达100%，该测定法耗时30分钟，灵敏度为40 IU/ml。与常规IgE测定法的相关系数r = 0.96，对10份血清进行测试的平均分析回收率为95.8%。