State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China.
Department of Orthopaedic, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
J Mater Chem B. 2024 Jun 27;12(25):6164-6174. doi: 10.1039/d4tb00506f.
Catalytic therapy based on nanozymes is promising for the treatment of bacterial infections. However, its therapeutic efficacy is usually restricted by the limited amount of hydrogen peroxide and the weak acidic environment in infected tissues. To solve these issues, we prepared polyvinyl alcohol (PVA)-polyacrylic acid (PAA)-iron oxide (FeO)/polyvinyl alcohol (PVA)-zinc peroxide (ZnO) double-layer electrospun nanofibers (PPF/PZ NFs). In this design, PVA serves as the carrier for ZnO nanoparticles (NPs), FeO NPs, and PAA. The double-layer structure of nanofibers can spatially separate the PAA and ZnO to avoid their reaction with each other during preparation and storage, while in the wet wound bed, PVA can dissolve and PAA can provide H ions to promote the generation of hydrogen peroxide and subsequent conversion to hydroxyl radicals for bacteria killing. experimental results demonstrated that PPF/PZ NFs can reduce the methicillin-resistant by 3.1 log (99.92%). Moreover, PPF/PZ NFs can efficiently treat the bacterial infection in a mouse wound model and promote wound healing with negligible toxicity to animals, indicating their potential use as "plug-and-play" antibacterial wound dressings. This work provides a novel strategy for the construction of double-layer electrospun nanofibers as catalytic wound dressings with hydrogen peroxide/acid self-supplying properties for the efficient treatment of bacterial infections.
基于纳米酶的催化疗法在治疗细菌感染方面很有前景。然而，其治疗效果通常受到感染组织中过氧化氢含量有限和弱酸性环境的限制。为了解决这些问题，我们制备了聚乙烯醇（PVA）-聚丙烯酸（PAA）-氧化铁（FeO）/聚乙烯醇（PVA）-过氧化锌（ZnO）双层静电纺丝纳米纤维（PPF/PZ NF）。在该设计中，PVA 作为 ZnO 纳米颗粒（NPs）、FeO NPs 和 PAA 的载体。纳米纤维的双层结构可以将 PAA 和 ZnO 空间分离，以避免它们在制备和储存过程中的相互反应，而在湿性伤口床上，PVA 可以溶解，PAA 可以提供 H 离子以促进过氧化氢的产生，随后转化为羟基自由基以杀死细菌。实验结果表明，PPF/PZ NF 可以使耐甲氧西林金黄色葡萄球菌减少 3.1 log（99.92%）。此外，PPF/PZ NF 可以有效地治疗小鼠伤口模型中的细菌感染，并促进伤口愈合，对动物几乎没有毒性，表明它们有潜力用作“即插即用”的抗菌伤口敷料。这项工作为构建具有过氧化氢/酸自供应性能的双层静电纺丝纳米纤维作为催化性伤口敷料提供了一种新策略，可有效治疗细菌感染。
