National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing, China.
College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.
Pest Manag Sci. 2024 Oct;80(10):4950-4958. doi: 10.1002/ps.8223. Epub 2024 Jun 3.
Dermanyssus gallinae, the poultry red mite (PRM), is a worldwide ectoparasite posing significant economic challenges in poultry farming. The extensive use of pyrethroids for PRM control has led to the emergence of pyrethroid resistance. The objective of this study is to detect the pyrethroid resistance and explore its associated point mutations in the voltage-gated sodium channel (VGSC) gene among PRM populations in China.
Several populations of D. gallinae, namely CJF-1, CJP-2, CJP-3, CSD-4 and CLD-5, displayed varying degrees of resistance to beta-cypermethrin compared to a susceptible field population (CBP-5). Mutations of VGSC gene in populations of PRMs associated with pyrethroid resistance were identified through sequencing its fragments IIS4-IIS5 and IIIS6. The mutations I917V, M918T/L, A924G and L925V were present in multiple populations, while no mutations were found at positions T929, I936, F1534 and F1538.
The present study confirmed the presence of extremely high levels of pyrethroid resistance in PRM populations in China, and for the first time detected four pyrethroid resistance mutations in the VGSC gene. Identifying pyrethroid resistance in the field population of PRM in China can be achieved through screening for VGSC gene mutations as an early detection method. Our findings underscore the importance of implementing chemical PRM control strategies based on resistance evidence, while also considering the management of acaricide resistance in the control of PRMs. © 2024 Society of Chemical Industry.
鸡皮刺螨（PRM）是一种世界性的外寄生虫，给家禽养殖业带来了重大的经济挑战。由于广泛使用拟除虫菊酯类药物来控制 PRM，导致了拟除虫菊酯类药物的抗药性。本研究的目的是检测中国 PRM 种群中拟除虫菊酯类药物的抗药性，并探讨其与电压门控钠离子通道（VGSC）基因相关的点突变。
与敏感田间种群（CBP-5）相比，几种鸡皮刺螨种群（CJF-1、CJP-2、CJP-3、CSD-4 和 CLD-5）对 beta-氯氰菊酯表现出不同程度的抗药性。通过对 PRM 种群 VGSC 基因片段 IIS4-IIS5 和 IIIS6 的测序，发现了与拟除虫菊酯类药物抗药性相关的基因突变。在多个种群中存在 I917V、M918T/L、A924G 和 L925V 突变，而在 T929、I936、F1534 和 F1538 位置未发现突变。
本研究证实了中国 PRM 种群存在极高水平的拟除虫菊酯类药物抗药性，并且首次在 VGSC 基因中检测到了四种拟除虫菊酯类药物抗药性突变。通过筛选 VGSC 基因突变，可以实现对中国 PRM 田间种群的抗药性检测，作为一种早期检测方法。本研究结果强调了根据抗药性证据实施化学 PRM 控制策略的重要性，同时还需要考虑在控制 PRM 时对抗螨剂抗药性的管理。
