assisted pharmacognosy of plant resources for discovering antivirulence molecules targeting .

The rise of antibiotic-resistant bacterial strains represents an important challenge for global health, underscoring the critical need for innovative strategies to confront this threat. Natural products and their derivatives have emerged as a promising reservoir for drug discovery. The social amoeba is a potent model organism in this effort. Employing this invertebrate model, we introduce a novel perspective to investigate natural plant extracts in search of molecules with potential antivirulence activity. Our work established an easy-scalable developmental assay targeting a virulent strain of , with as the representative plant. The main objective was to identify tentative compounds from the extract that attenuate the virulence of virulence without inducing cytotoxic effects on amoeba cells. Notably, the methanolic root extract of fulfilled these prerequisites compared to the dichloromethane extract. Using UHPLC Q/Orbitrap/ESI/MS/MS, 63 compounds were tentatively identified in both extracts, 47 in the methanolic and 29 in the dichloromethane, with 13 compounds in common. This research underscores the potential of employing -assisted pharmacognosy to discover new antivirulence agents against multidrug-resistant pathogens.