Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture; College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, China.
Qingdao Greensum Ecology Co., Ltd., Qingdao 266102, China.
J Appl Microbiol. 2024 Jun 3;135(6). doi: 10.1093/jambio/lxae132.
We investigated the effects of the aggregate spray-seeding (ASS) technique on soil bacterial community diversity, life strategies, and seasonal change.
Soil from six plots with original vegetation (CK, n = 6) was compared to soil from 15 plots with spray-seeding restoration (SR, n = 15) using environmental DNA sequencing. The bacterial Shannon and Chao1 indices of SR soils were significantly greater (P < 0.05) than those of CK soils. The Chao1 index for the SR soil bacterial community was significantly greater in summer (P < 0.05) than in winter. The ratio of the relative abundance of bacterial K-strategists to r-strategists (K/r) and the DNA guanine-cytosine (GC) content in the SR soil were significantly lower (P < 0.05) than those in the CK soil. Principal coordinate analysis revealed significant differences between the SR and CK bacterial communities. The GC content was positively correlated with the K/r ratio. Soil conductivity was negatively associated with the K/r ratio and GC content, indicating that ionic nutrients were closely related to bacterial life strategies.
The ASS technique improved soil bacterial diversity, altered community composition, and favored bacterial r-strategists.
本研究旨在探讨集束喷播（ASS）技术对土壤细菌群落多样性、生活策略和季节变化的影响。
采用环境 DNA 测序的方法，比较了 6 个具有原始植被的样本（CK，n=6）和 15 个具有喷播恢复植被的样本（SR，n=15）土壤中的细菌群落多样性。SR 土壤的细菌 Shannon 和 Chao1 指数明显大于 CK 土壤（P<0.05）。SR 土壤的细菌群落 Chao1 指数在夏季显著大于冬季（P<0.05）。SR 土壤中细菌 K-策略者相对丰度与 r-策略者相对丰度的比值（K/r）和 DNA 鸟嘌呤-胞嘧啶（GC）含量明显低于 CK 土壤（P<0.05）。主坐标分析显示，SR 和 CK 土壤的细菌群落存在显著差异。GC 含量与 K/r 比值呈正相关。土壤电导率与 K/r 比值和 GC 含量呈负相关，表明离子养分与细菌的生活策略密切相关。
ASS 技术提高了土壤细菌的多样性，改变了群落组成，并有利于细菌的 r-策略者。
