Numata O, Sugai T, Watanabe Y
Nature. 1985;314(6007):192-4. doi: 10.1038/314192a0.
Intermediate filament protein [relative molecular mass (Mr) 49,000 (49K)] from the ciliated protozoan Tetrahymena has been shown to resemble intermediate filament proteins from mammalian cells in several respects, and to have a possible role in the oral morphogenesis preceding binary fission in Tetrahymena. Here, based on immunofluorescence localization of the 49K protein in Tetrahymena during the early stages of conjugation, we suggest that the protein is involved in some nuclear events, such as the production of four haploid nuclei by prezygotic divisions (meiosis), selection of one of the four meiotic products, formation of the gametic pronucleus by the mitotic division of the selected meiotic product, transfer of the gametic pronucleus across a cell-cell junction, and zygote formation by pronuclear fusion.
来自纤毛原生动物四膜虫的中间丝蛋白[相对分子质量(Mr) 49,000 (49K)]已被证明在几个方面类似于哺乳动物细胞的中间丝蛋白，并且在四膜虫二分裂之前的口部形态发生中可能发挥作用。在此，基于在接合早期四膜虫中49K蛋白的免疫荧光定位，我们认为该蛋白参与了一些核事件，例如通过合子前分裂（减数分裂）产生四个单倍体核、从四个减数分裂产物中选择一个、通过所选减数分裂产物的有丝分裂形成配子原核、配子原核通过细胞间连接转移以及通过原核融合形成合子。
