Messmer E P, Font R L, Kirkpatrick J B, Höpping W
Ophthalmology. 1985 Jan;92(1):167-73. doi: 10.1016/s0161-6420(85)34076-9.
Sections of 51 surgically enucleated eyes from cases of retinoblastomas were examined immunohistochemically to delineate patterns of cellular differentiation. Employing the avidin-biotin immunoperoxidase technique, antibodies were used against glial fibrillary acidic protein (GFAP), S-100 protein and neuron specific enolase (NSE). Areas of uninvolved retina and/or optic nerve were used as built-in positive control. Most of the tumors showed GFAP and S-100 protein-positive perivascular glial cells that were interpreted as reactive astrocytes. In three well-differentiated retinoblastomas, glial cells were found to be interspersed randomly among tumor cells and not associated with blood vessels. These glial cells were interpreted as neoplastic based on their distribution pattern and cytologic features. In about half of the tumors, the retinoblastoma cells stained positively for NSE, indicating their neuronal tumor cells that stained positively for NSE but failed to show any evidence of photoreceptor differentiation. Based on these observations and similar differentiation patterns described in other primitive neuroectodermal tumors of the brain, it is suggested that retinoblastoma cells can differentiate not only into photoreceptor cells but also along other neuronal cell lines and rarely into glial cells.
对51例视网膜母细胞瘤手术摘除眼球的切片进行免疫组化检查，以描绘细胞分化模式。采用抗生物素蛋白-生物素免疫过氧化物酶技术，使用针对胶质纤维酸性蛋白（GFAP）、S-100蛋白和神经元特异性烯醇化酶（NSE）的抗体。未受累的视网膜和/或视神经区域用作内对照阳性对照。大多数肿瘤显示GFAP和S-100蛋白阳性的血管周围胶质细胞，被解释为反应性星形胶质细胞。在三个高分化视网膜母细胞瘤中，发现胶质细胞随机散布在肿瘤细胞之间，且与血管无关。基于其分布模式和细胞学特征，这些胶质细胞被解释为肿瘤性的。在大约一半的肿瘤中，视网膜母细胞瘤细胞NSE染色阳性，表明它们是神经元肿瘤细胞，NSE染色阳性但未显示任何光感受器分化的证据。基于这些观察结果以及在脑的其他原始神经外胚层肿瘤中描述的类似分化模式，提示视网膜母细胞瘤细胞不仅可以分化为光感受器细胞，还可以沿着其他神经元细胞系分化，很少分化为胶质细胞。
