Department of Chronic Non-Communicable Disease Control, Changzhou Center for Disease Control and Prevention, Changzhou Advanced Institute of Public Health, Nanjing Medical University, No. 203 Taishan Road, Xinbei District, Changzhou, 213022, Jiangsu, People's Republic of China.
Department of Chronic Non-Communicable Disease Control, Changzhou Wujin District Disease Prevention and Control Center, Changzhou, 213164, Jiangsu, People's Republic of China.
Acta Diabetol. 2024 Nov;61(11):1423-1432. doi: 10.1007/s00592-024-02309-x. Epub 2024 Jun 4.
We aimed to evaluate the impact of C-reactive protein (CRP) gene polymorphism, additional gene-gene interaction, and haplotypes on susceptibility to type 2 diabetes mellitus (T2DM).
SNPstats online software ( https://www.snpstats.net/start.htm ) was employed to evaluate the association between CRP gene and T2DM risk. High-order interactions among SNPs was tested using generalized multifactor dimensionality reduction, and the testing balanced accuracy, training balanced accuracy and cross-validation consistency were calculated. The SHEsisPlus ( http://shesisplus.bio-x.cn/SHEsis.html ) online software was used for haplotype analysis.
A total of 730 T2DM patients and 765 controls were enrolled. The T allele of rs1205 is associated with increased susceptibility to T2DM, OR (95% CI) were 1.51 (1.13-2.01), 1.44 (1.10-1.89) and 1.25 (1.01-1.54) for codominant, dominant and over-dominant models, respectively. We also found that minor allele of rs2794521 is associated with decreased susceptibility to T2DM under codominant and recessive models, OR (95%CI) were 0.38 (0.18-0.79) and 0.37 (0.16-0.80) for codominant and recessive models, respectively. No significant gene-gene interaction existed among CRP gene SNPs, all interaction p- values were more than 0.05. Haplotype analyses suggested the CGCA haplotype containing rs1205-C, rs1130864-G, rs2794521- C and rs3093059- A allele was associated with decreased risk of T2DM, OR (95% CI) = 0.83 (0.68-0.98), P = 0.047.
Minor allele of rs1205 was associated with increased T2DM risk. Minor allele of rs2794521 and the CGCA haplotype were associated with decreased T2DM risk.
本研究旨在评估 C 反应蛋白（CRP）基因多态性、额外的基因-基因相互作用以及单倍型对 2 型糖尿病（T2DM）易感性的影响。
使用 SNPstats 在线软件（https://www.snpstats.net/start.htm）评估 CRP 基因与 T2DM 风险之间的关联。采用广义多因子降维法检验 SNP 之间的高阶相互作用，并计算检验平衡准确性、训练平衡准确性和交叉验证一致性。使用 SHEsisPlus（http://shesisplus.bio-x.cn/SHEsis.html）在线软件进行单倍型分析。
共纳入 730 例 T2DM 患者和 765 例对照。rs1205 的 T 等位基因与 T2DM 的易感性增加相关，在共显性、显性和过显性模型中，OR（95%CI）分别为 1.51（1.13-2.01）、1.44（1.10-1.89）和 1.25（1.01-1.54）。我们还发现，rs2794521 的次要等位基因与共显性和隐性模型下 T2DM 的易感性降低相关，OR（95%CI）分别为 0.38（0.18-0.79）和 0.37（0.16-0.80）。CRP 基因 SNP 之间不存在显著的基因-基因相互作用，所有交互 p 值均大于 0.05。单倍型分析表明，包含 rs1205-C、rs1130864-G、rs2794521-C 和 rs3093059-A 等位基因的 CGCA 单倍型与 T2DM 的发病风险降低相关，OR（95%CI）=0.83（0.68-0.98），P=0.047。
rs1205 的次要等位基因与 T2DM 风险增加相关。rs2794521 的次要等位基因和 CGCA 单倍型与 T2DM 风险降低相关。
