Department of Biotechnology, College of Natural and Applied Science, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia.
Department of Biology, College of Natural and Computational Sciences, Wolaita Sodo University, Wolaita Sodo, Ethiopia.
Virus Genes. 2024 Aug;60(4):423-433. doi: 10.1007/s11262-024-02077-4. Epub 2024 Jun 4.
White yam (Dioscorea rotundata) plants collected from farmers' fields and planted at the Areka Agricultural Research Center, Southern Ethiopia, displayed mosaic, mottling, and chlorosis symptoms. To determine the presence of viral pathogens, an investigation for virome characterization was conducted by Illumina high-throughput sequencing. The bioinformatics analysis allowed the assembly of five viral genomes, which according to the ICTV criteria were assigned to a novel potyvirus (3 genome sequences) and a novel crinivirus (2 genome sequences). The potyvirus showed ~ 66% nucleotide (nt) identity in the polyprotein sequence to yam mosaic virus (NC004752), clearly below the demarcation criteria of 76% identity. For the crinivirus, the RNA 1 and RNA 2 shared the highest sequence identity to lettuce chlorosis virus, and alignment of the aa sequence of the RdRp, CP and HSP70h (~ 49%, 45% and 76% identity), considered for the demarcation criteria, revealed the finding of a novel virus species. The names Ethiopian yam virus (EYV) and Yam virus 1 (YV-1) are proposed for the two tentative new virus species.
从埃塞俄比亚南部 Areka 农业研究中心农民田间收集的白薯（Dioscorea rotundata）植物表现出花叶、斑驳和黄化症状。为了确定是否存在病毒病原体，通过 Illumina 高通量测序进行了病毒组特征调查。生物信息学分析允许组装五个病毒基因组，根据 ICTV 标准，这些基因组被分配给一种新型马铃薯 Y 花叶病毒（NC004752）和一种新型蕨叶病毒（2 个基因组序列）。在多蛋白序列中，马铃薯 Y 花叶病毒的核苷酸（nt）同一性约为 66%，明显低于 76%的同一性判别标准。对于蕨叶病毒，RNA1 和 RNA2 与生菜褪绿病毒的序列同一性最高，RdRp、CP 和 HSP70h 的 aa 序列比对（~49%、45%和 76%的同一性），考虑到判别标准，发现了一种新型病毒。拟议将这两个暂定新病毒种命名为埃塞俄比亚薯病毒（EYV）和薯病毒 1（YV-1）。
