Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, China.
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, China; Analysis and Testing Center, NERC Biomass of Changzhou University, Jiangsu, 213032, China.
Anal Chim Acta. 2024 Jul 11;1312:342763. doi: 10.1016/j.aca.2024.342763. Epub 2024 May 22.
Developing effective electrochemiluminescence (ECL) platforms is always an essential concern in highly sensitive bioanalysis. In this work, a low-triggering-potential ECL sensor was designed for detecting synthetic cathinone 3,4-methylenedioxypyrovalerone (MDPV) based on a dual-signal amplification strategy. Initially, a probe was created by integrating Ruthenium into the hollow porphyrin-based MOF (PCN-222) structure to decrease the excitation potential and enhance ECL performance without external co-reaction accelerators. Additionally, for the first time, photonic crystals (PCs) assembled from covalent organic frameworks (COFs) were employed to amplify the ECL signal, thereby increasing the photon flux and the loading capacity of the ECL emitter to enhance sensitivity of the sensor. In the presence of the target MDPV, the aptamer labeled with Ferrocene (Fc) experienced conformational changes, causing Fc to approach the luminophore and resulting in ECL quenching. This effect was attributed to aptamer's conformational changes induced by the target, directly correlating with the target concentration. The constructed sensor showed good linearity with the target MDPV concentration, covering a dynamic range from 1.0 × 10 to 1.0 × 10 g/L and achieved an ultra-low detection limit of 4.79 × 10 g/L. This work employed dual amplification strategies to enhance ECL signals effectively, providing a novel method for developing highly responsive and bioactive sensors.
开发有效的电致化学发光（ECL）平台一直是高灵敏度生物分析中至关重要的关注点。在这项工作中，设计了一种低触发电位的 ECL 传感器，用于基于双重信号放大策略检测合成卡西酮 3,4-亚甲二氧基吡咯戊酮（MDPV）。首先，通过将钌整合到基于中空卟啉的 MOF（PCN-222）结构中，创建了一个探针，以降低激发电位并增强 ECL 性能，而无需外部共反应加速剂。此外，首次使用由共价有机框架（COFs）组装的光子晶体（PCs）来放大 ECL 信号，从而增加了光子通量和 ECL 发射器的负载能力，从而提高了传感器的灵敏度。在存在目标 MDPV 的情况下，标记有二茂铁（Fc）的适体经历构象变化，导致 Fc 接近发光体，从而导致 ECL 猝灭。这种效应归因于目标诱导的适体构象变化，与目标浓度直接相关。所构建的传感器对目标 MDPV 浓度表现出良好的线性关系，涵盖了 1.0×10 至 1.0×10 g/L 的动态范围，并实现了超低检测限为 4.79×10 g/L。这项工作采用了双重放大策略来有效增强 ECL 信号，为开发高响应性和生物活性传感器提供了一种新方法。
