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通过简单检测血清花生四烯酸水平来辅助病毒性感染的管理。

Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level.

机构信息

Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Pza. de las Ciencias 2, Madrid, 28040, Spain.

Servei de Medicina Interna, Hospital Universitari de Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Av. Dr. Josep Laporte 2, Reus, 43204, Spain.

出版信息

Mikrochim Acta. 2024 Jun 4;191(7):369. doi: 10.1007/s00604-024-06440-y.

DOI:10.1007/s00604-024-06440-y
PMID:38834823
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11150294/
Abstract

A trendsetting direct competitive-based biosensing tool has been developed and implemented for the determination of the polyunsaturated fatty acid arachidonic acid (ARA), a highly significant biological regulator with decisive roles in viral infections. The designed methodology involves a competitive reaction between the target endogenous ARA and a biotin-ARA competitor for the recognition sites of anti-ARA antibodies covalently attached to the surface of carboxylic acid-coated magnetic microbeads (HOOC-MµBs), followed by the enzymatic label of the biotin-ARA residues with streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The resulting bioconjugates were magnetically trapped onto the sensing surface of disposable screen-printed carbon transducers (SPCEs) to monitor the extent of the biorecognition reaction through amperometry. The operational functioning of the exhaustively optimized and characterized immunosensing bioplatform was highly convenient for the quantitative determination of ARA in serum samples from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-) and respiratory syncytial virus (RSV)-infected individuals in a rapid, affordable, trustful, and sensitive manner.

摘要

一种开创性的直接竞争型生物传感工具已经被开发并应用于测定多不饱和脂肪酸花生四烯酸（ARA），这是一种具有重要生物学调节作用的物质，在病毒感染中具有决定性作用。所设计的方法涉及到目标内源性 ARA 与生物素-ARA 竞争物之间的竞争反应，用于识别与共价附着在羧酸涂层磁性微珠（HOOC-MµBs）表面的抗 ARA 抗体的识别位点，随后用链霉亲和素-辣根过氧化物酶（Strep-HRP）缀合物对生物素-ARA 残基进行酶标记。所得的生物缀合物通过磁捕获到一次性使用的丝网印刷碳传感器（SPCEs）的传感表面上，通过电流测定法监测生物识别反应的程度。经过彻底优化和表征的免疫传感生物平台的操作功能非常方便，可用于快速、经济、可靠和敏感地定量测定严重急性呼吸综合征冠状病毒 2（SARS-CoV-2-）和呼吸道合胞病毒（RSV）感染个体血清样本中的 ARA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/d136728895c2/604_2024_6440_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/ad10ffe7edb0/604_2024_6440_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/1a528e948943/604_2024_6440_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/9af136f5c2be/604_2024_6440_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/cbd35af24808/604_2024_6440_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/d136728895c2/604_2024_6440_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/ad10ffe7edb0/604_2024_6440_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/1a528e948943/604_2024_6440_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/9af136f5c2be/604_2024_6440_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/cbd35af24808/604_2024_6440_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a272/11150294/d136728895c2/604_2024_6440_Fig5_HTML.jpg

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