Canine peripheral non-conventional TCRαβ CD4CD8α double-negative T cells show T helper 2-like and regulatory properties.

The dog is an important companion animal and also serves as model species for human diseases. Given the central role of T cells in immune responses, a basic understanding of canine conventional T cell receptor (TCR)αβ T cells, comprising CD4 single-positive (sp) T helper (Th) and CD8α sp cytotoxic T cell subsets, is available. However, characterization of canine non-conventional TCRαβ CD4CD8α double-positive (dp) and TCRαβ CD4CD8α double-negative (dn) T cells is limited. In this study, we performed a comprehensive analysis of canine dp and dn T cells in comparison with their conventional counterparts. TCRαβ T cells from peripheral blood of healthy dogs were sorted according to their CD4/CD8α phenotype into four populations (i.e. CD4 sp, CD8α sp, dp, and dn) and selected surface markers, transcription factors and effector molecules were analyzed and after stimulation by RT-qPCR. Novel characteristics of canine dp T cells were identified, expanding the previously characterized Th1-like phenotype to Th17-like and Th2-like properties. Overall, mRNA expression of various Th cell-associated cytokines (i.e. ) in dp T cells upon stimulation highlights their versatile immunological potential. Furthermore, we demonstrated that the CD4CD8α dn phenotype is stable during stimulation. Strikingly, dn T cells were found to express highest mRNA levels of type 2 effector cytokines () upon stimulation. Their strong ability to produce IL-4 was confirmed at the protein level. Upon stimulation, the percentage of IL-4-producing cells was even higher in the non-conventional dn than in the conventional CD4 sp population. Constitutive transcription of (encoding IL-33Rα) further supports Th2-like properties within the dn T cell population. These data point to a role of dn T cells in type 2 immunity. In addition, the high potential of dn T cells to transcribe the gene encoding the co-inhibitory receptor CTLA-4 and to produce the inhibitory cytokine IL-10 indicates putative immunosuppressive capacity of this population. In summary, this study reveals important novel aspects of canine non-conventional T cells providing the basis for further studies on their effector and/or regulatory functions to elucidate their role in health and disease.