Department of Burn and Plastic Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu, China.
Department of Burn and Plastic Surgery, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215000, Jiangsu, China.
Cell Mol Biol (Noisy-le-grand). 2024 Jun 5;70(6):21-27. doi: 10.14715/cmb/2024.70.6.4.
This research aimed to investigate the effect of slow-released angiogenin by silicon micro-needle on angiogenesis in the Choke zone of dorsal multiple-territory perforator flap in rats, as well as its mechanism. Thirty-six adult Sprague-Dawley (SD) rats were randomly divided into control group, model group, and four experimental groups. In model group, slow-release saline through a silicon micro-needle was placed in choke II zone of the flap 7 days before the operation. For rats in four experimental groups, angiogenin was released via micro-needle in the choke I and choke II zones of the cross-zone flap 7 days before and 3 days before flap surgery, respectively. A 12 cm × 3 cm cross-zone perforator flap model was made on the back of all five groups. The flap survival rate in slow-release angiopoietin group was statistically higher than that in model group (P<0.05). Angiogenin in choke zone of the flap was increased in slow-release angiogenin group (P<0.05). In slow-release angiogenin group, the micro-vessel density was increased and the arteriovenous diameter was decreased, while the arteriovenous diameter was increased in model group (P<0.05). The levels of vascular endothelial growth factor A (VEGF-A) and angiotensin 1 (ANG-1) in choke zone were both elevated in slow-release angiogenin group (P<0.05). The expression of CD31 was significantly elevated in flaps of experimental groups (P<0.05). Micro-needle to slow release Angiogenin can increase the drug concentration in the tissues of the choke zone, promote the vascularization of rat dorsal crossover area perforator flap, reduce the possibility of flap ischemic necrosis, and improve the flap survival rate.
本研究旨在探讨硅制微针缓释血管生成素对大鼠背部分叉多穿支皮瓣梗塞区血管生成的影响及其作用机制。将 36 只成年 Sprague-Dawley（SD）大鼠随机分为对照组、模型组和四个实验组。模型组于术前 7 天在皮瓣梗塞 II 区通过硅制微针缓慢释放生理盐水。四个实验组分别于术前 7 天和皮瓣手术前 3 天在跨区皮瓣梗塞 I 区和梗塞 II 区通过微针释放血管生成素。在五组大鼠背部均制作 12cm×3cm 的跨区穿支皮瓣模型。缓释血管生成素组皮瓣存活率明显高于模型组（P<0.05）。缓释血管生成素组梗塞区血管生成素含量增加（P<0.05）。缓释血管生成素组微血管密度增加，动静脉直径减小，而模型组动静脉直径增加（P<0.05）。缓释血管生成素组梗塞区血管内皮生长因子 A（VEGF-A）和血管紧张素 1（ANG-1）水平均升高（P<0.05）。实验组皮瓣 CD31 表达明显升高（P<0.05）。微针缓释血管生成素可增加梗塞区组织内药物浓度，促进大鼠背部分叉穿支皮瓣血管化，减少皮瓣缺血性坏死的可能性，提高皮瓣存活率。
