Department of Orthopaedics Surgery, Wuxi No.9 People's Hospital Affiliated to Soochow University, Wuxi, 214000, China.
Department of Orthopaedics Surgery, Wuxi No.9 People's Hospital Affiliated to Soochow University, Wuxi, 214000, China.
Injury. 2020 Nov;51(11):2442-2448. doi: 10.1016/j.injury.2020.07.058. Epub 2020 Jul 27.
The present study was designed to investigate the effects of irisin pre-treatment on multi-territory perforator flap survival and blood vessels of Choke II zone in rats and to explore the underlying mechanism.
Eighty adult Sprague-Dawley (SD) rats were divided into the Irisin group and the saline group (N = 40 each), and subjected to multi-territory perforator flap operations on the left flank. Rats in the irisin group were intraperitoneally injected with irisin (40 ng/kg), and rats in the control group were intraperitoneally injected with normal saline. Seven days after operation, we calculated the surviving flap area was calculated, and measured the density of microvessels, epidermal growth factor and blood flow in Choke II zone.
Irisin pre-treatment could improve the flap survival area, increase average density of microvessels and up-regulate the mRNA and protein level of vascular endothelial growth factor, thereby promoting flap angiogenesis.
Irisin improved flap blood supply and increase the survival rate of multi-territory perforator flap by promoting angiogenesis of flank multi-territory perforator flap survival in Choke II zone.
本研究旨在探讨鸢尾素预处理对大鼠多区域穿支皮瓣存活和 II 区梗塞带血管的影响，并探讨其潜在机制。
80 只成年 Sprague-Dawley（SD）大鼠分为鸢尾素组和生理盐水组（每组 40 只），并在左侧侧腹进行多区域穿支皮瓣手术。鸢尾素组大鼠腹腔内注射鸢尾素（40ng/kg），对照组大鼠腹腔内注射生理盐水。术后 7 天，计算存活皮瓣面积，并测量 II 区梗塞带微血管密度、表皮生长因子和血流。
鸢尾素预处理可提高皮瓣存活面积，增加微血管平均密度，并上调血管内皮生长因子的 mRNA 和蛋白水平，从而促进皮瓣血管生成。
鸢尾素通过促进侧腹多区域穿支皮瓣 II 区梗塞带的血管生成，改善皮瓣血供，提高多区域穿支皮瓣的成活率。
